The Chlamydomonas Sourcebook Introduction to Chlamydomonas and Its Laboratory Use Volume 1 Second Edition by Elizabeth Harris, Elizabeth H Harrisduke ISBN 9780123708748 0123708745

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Product details: 

ISBN 10:  0123708745

ISBN 13: 9780123708748

Author:  Elizabeth H. Harris, Elizabeth H Harrisduke

Dr. Harris has played a major role in the development of this organism as a model system. Her previous version of the Chlamydomonas Sourcebook which published in 1989, has been a classic in the field and is considered required reading for anyone working with this organism. This latest edition has been expanded to include three volumes providing molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism. Methods for Chlamydomonas research and best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media, are included. Additions to this volume also include help with common laboratory problems such as contamination, student demonstrations, and properties of particular strains and mutants.

This volume is part of a 3-Volume Set (ISBN: 978-0-12-370873-1) and is also sold individually.

Expanded revision of gold standard reference
Includes latest advances in research, including completion of the genome
Provides broad perspective with studies in cell and molecular biology, genetics, plant physiology and related fields
Available as part of a 3-Volume Set or sold individually

Table of contents: 

CHAPTER 1

The Genus Chlamydomonas..

1. Introduction

II. Description of the genus

III. Chlamydomonas genetics: 1830-1960..

IV. Origins of the major laboratory strains of

C. reinhardtii…

V. Other isolates of C. reinhardtii

VI. Other Chlamydomonas species used experimentally.

CHAPTER 2

Cell Architecture

1. Introduction

II. Early ultrastructural studies.

III. The cell wall

IV. Basal bodies, flagellar roots, and cellular filaments…..

V. The nucleus..

VI. Golgi and endoplasmic reticulum

VII. Contractile vacuoles..

VIII. Microbodies and other membrane-bound structures

IX. Mitochondria

X. The chloroplast

XI. The eyespot

CHAPTER 3 Cell Division

1. Introduction

II. Mitosis.

III. Meiosis

IV. Chromosome cytology

V. The cell cycle

CHAPTER 4 Motility and Behavior.

1. Introduction

II. Flagellar structure and organization

III. Assembly and maintenance of the flagella

IV. Motility

V. Cell behavior

CHAPTER 5 The Sexual Cycle.

1. Introduction

II. Types of sexual reproduction within the genus Chlamydomonas…

III. Stages in the reproductive process.

IV. Vegetative diploids and cytoduction

V. Genetic control of sexuality

VI. Perturbations of the mating process.

CHAPTER 6 The Life of an Acetate Flagellate

1. Introduction

II. Nucleic acids and protein synthesis

III. Metabolic processes

IV. Photosynthesis.

V. Respiration…..

VI. Hydrogen production

CHAPTER 7 Organelle Heredity.

1. Introduction

II. Uniparental inheritance

III. Organelle DNA..

IV. RNA and protein synthesis in the chloroplast

V. Coordination of nuclear and organelle genomes in organelle biogenesis

CHAPTER 8 Chlamydomonas in the Laboratory.

1. Introduction

II. Culture media..

III. Culture conditions.

IV. Harvesting and fractionating cultures.

V. Histological techniques.

VI. Isolation of Chlamydomonas species from nature..

VII. Storage of Chlamydomonas strains

VIII. Genetic analysis.

IX. The molecular toolkit

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