T follicular Helper Cells Methods and Protocols 1st Edition by Marion Espéli, Michelle Linterman ISBN 978-1493955930 ‎ 1493955934

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Product details: 

ISBN 10:   1493955934

ISBN 13: 978-1493955930 ‎

Author: Marion Espéli, Michelle Linterman

This volume brings together the skills and protocols of numerous laboratories that are at the heart of investigation into the biology of Tfh cells in both mice and humans. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

Concise and easy-to-use, T follicular Helper Cells: Methods and Protocols provides scientist with techniques and protocols that have facilitated breakthroughs in Tfh biology and to present them in a way that will enable both new and experienced resear

Table of contents: 

PART I: MOUSE

1. Identification of Mouse T Follicular Helper Cells by Flow Cytometry – Alexandre P. Meli and Irah L. King

2. Identification of Follicular T Helper Cells in Tissue Sections – Yanna Ding, John D. Mountz, and Hui-Chen Hsu

3. Tracking Early T Follicular Helper Cell Differentiation In Vivo – Dirk Baumjohann and K. Mark Ansel

4. Tracking by Flow Cytometry Antigen-Specific Follicular Helper T Cells in Wild-Type Animals After Protein Vaccination – Svetoslav Chakarov and Nicolas Fazilleau

5. Retroviral Vector Expression in TCR Transgenic CD4+ T Cells – Youn Soo Choi and Shane Crotty

6. Two-Photon Microscopy for Imaging Germinal Centers and T Follicular Helper Cells – Menna R. Clatworthy

7. In Vivo Induction of T-Follicular Helper Cells by Modulation of Regulatory T Cell Function – James Badger Wing and Shimon Sakaguchi

8. Assessing T Follicular Helper Cell Function In Vivo: Antigen-Specific B Cell Response to Hapten and Affinity Maturation – Jessica Natt and Marion Espéli

9. The SWHEL System for High-Resolution Analysis of In Vivo Antigen-Specific T-Dependent B Cell Responses – R. Brink, D. Paus, K. Bourne, J.R. Hermes, S. Gardam, T.G. Phan, and T.D. Chan

10. Triggering Positive Selection of Germinal Center B Cells by Antigen Targeting to DEC-205 – Giulia Pasqual, Alessandro Angelini, and Gabriel D. Victora

11. Detection of Mouse Natural Killer T Follicular Helper (NKTFH) Cells by Flow Cytometry – Pheh-Ping Chang and Carola G. Vinuesa

12. Identification of Foxp3+ T Follicular Regulatory (Tfr) Cells by Flow Cytometry – Ana Raquel Maceiras and Luis Graca

13. In Vitro Assay to Sensitively Measure TFR Suppressive Capacity and TFH Stimulation of B Cell Responses – Peter T. Sage and Arlene H. Sharpe

PART II: HUMAN

14. Flow Cytometric Detection and Isolation of Human Tonsil or Lymph Node T Follicular Helper Cells – Jan Misiak, Karin Tarte, and Patricia Amé-Thomas

15. Human Tfh and Tfr Cells: Identification and Assessment of Their Migration Potential – Chang H. Kim, Seika Hashimoto-Hill, and Seung G. Kang

16. Analysis of Human Blood Memory T Follicular Helper Subsets – Salah-Eddine Bentebibel, Clement Jacquemin, Nathalie Schmitt, and Hideki Ueno

17. Flow Cytometric Analysis of Circulating Follicular Helper T (Tfh) and Follicular Regulatory T (Tfr) Populations in Human Blood – Yunbo Wei, Jinhong Feng, Zhaohua Hou, Xin Maggie Wang, and Di Yu

18. Quantifying Helper Cell Function of Human TFH Cells In Vitro – Nina Chevalier

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Tags: Marion Espéli, Michelle Linterman, T follicular, Helper Cells