Sirtuins Methods and Protocols 1st Edition by Leonard Guarente, Matthew Hirschey ISBN 9781627036375 1627036377

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Product details: 

ISBN 10: 1627036377

ISBN 13: 9781627036375

Author:  Leonard Guarente, Matthew D. Hirschey

Table of contents: 

Methods to Generate Sirtuin Biology Tools
1 Front Matter
2 Download chapter PDF
3 Introduction: Sirtuins in Aging and Diseases
Leonard Guarente
4 Sirtuins in Yeast: Phenotypes and Tools
Scott Tsuchiyama, Elizabeth Kwan, Weiwei Dang, Antonio Bedalov, Brian K. Kennedy
5 C. elegans Sirtuins
Mohan Viswanathan, Heidi A. Tissenbaum
6 Genetic and Biochemical Tools for Investigating Sirtuin Function in Drosophila melanogaster
Jason G. Wood, Rachel Whitaker, Stephen L. Helfand
7 Generating Mammalian Sirtuin Tools for Protein-Interaction Analysis
Kathleen A. Hershberger, Jonathan Motley, Matthew D. Hirschey, Kristin A. Anderson

Methods to Identify Sirtuin Substrates
8 Front Matter
9 Download chapter PDF
10 Mass Spectrometry-Based Detection of Protein Acetylation
Yu Li, Jeffrey C. Silva, Mary E. Skinner, David B. Lombard
11 SILAC-Based Quantification of Sirt1-Responsive Lysine Acetylome
Yue Chen, Gozde Colak, Yingming Zhao
12 Targeted Quantitation of Acetylated Lysine Peptides by Selected Reaction Monitoring Mass Spectrometry
Matthew J. Rardin, Jason M. Held, Bradford W. Gibson
13 Identification of Deacetylase Substrates with the Biotin Switch Approach
J. Will Thompson, Alex Robeson, Joshua L. Andersen
14 Assaying Chromatin Sirtuins
Lei Zhong, Barbara Martinez-Pastor, Dafne M. Silberman, Carlos Sebastian, Raul Mostoslavsky

Methods to Measure Sirtuin Activity
15 Front Matter
16 Download chapter PDF
17 Measurement of Sirtuin Enzyme Activity Using a Substrate-Agnostic Fluorometric Nicotinamide Assay
Basil P. Hubbard, David A. Sinclair
18 Detecting Sirtuin-Catalyzed Deacylation Reactions Using 32P-Labeled NAD and Thin-Layer Chromatography
Anita Zhu, Xiaoyang Su, Hening Lin
19 Chemical Acetylation and Deacetylation
Kristofer S. Fritz
20 Accurate Measurement of Nicotinamide Adenine Dinucleotide (NAD+) with High-Performance Liquid Chromatography
Jun Yoshino, Shin-ichiro Imai
21 In Vivo Measurement of the Acetylation State of Sirtuin Substrates as a Proxy for Sirtuin Activity
John Dominy, Pere Puigserver, Carles Cantó
22 Methods to Study Sirtuin Biology

Methods to Study Sirtuin Biology
1 Oxygen Flux Analysis to Understand the Biological Function of Sirtuins
Dongning Wang, Michelle F. Green, Eoin McDonnell, Matthew D. Hirschey
2 The Emerging Links Between Sirtuins and Autophagy
In Hye Lee, Jeanho Yun, Toren Finkel
3 Methods to Study the Role of Sirtuins in Genome Stability
Paloma Martínez-Redondo, Alejandro Vaquero
4 Circadian Measurements of Sirtuin Biology
Kathryn Moynihan Ramsey, Alison H. Affinati, Clara B. Peek, Biliana Marcheva, Hee-Kyung Hong, Joseph Bass
5 Utilizing Calorie Restriction to Evaluate the Role of Sirtuins in Healthspan and Lifespan of Mice
Jessica Curtis, Rafael de Cabo
6 Erratum: Sirtuins: Methods and Protocols

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