Single Nucleotide Polymorphisms Methods and Protocols 2nd Edition by Barkur Shastry, Anton Komar ISBN 9781603274111, 1603274111

Single Nucleotide Polymorphisms Methods and Protocols 2nd Edition by Barkur S. Shastry, Anton A. Komar – Ebook PDF Instant Download/Delivery: 9781603274111, 1603274111
Full download Single Nucleotide Polymorphisms Methods and Protocols 2nd Edition after payment

Product details: 

ISBN 10: 1603274111

ISBN 13: 9781603274111

Author: Barkur S. Shastry, Anton A. Komar

Single Nucleotide Polymorphisms: Methods and Protocols (2nd Edition), edited by Anton A. Komar with contributions from Barkur S. Shastry, is a comprehensive laboratory manual in the Methods in Molecular Biology series. It provides detailed protocols and methodologies for the detection, analysis, and interpretation of single nucleotide polymorphisms (SNPs). Covering both bioinformatics and experimental approaches—including genotyping techniques like mass spectrometry, real-time PCR, and nanopore sequencing—it serves as a practical guide for researchers in genetics, molecular biology, and biomedical sciences.

Table of contents: 

SECTION I: INTRODUCTION TO SINGLE NUCLEOTIDE POLYMORPHISMS

1. SNPs: Impact on Gene Function and Phenotype Barkur S. Shastry

2. Silent (Synonymous) SNPs: Should We Care About Them?.

Ryan Hunt, Zuben E. Sauna, Suresh V. Ambudkar, Michael M. Gottesman, and Chava Kimchi-Sarfaty

SECTION II: BIOINFORMATIC ANALYSIS OF SNPS

3. SNP Databases

Christopher Phillips

4. Mining SNPs from DNA Sequence Data; Computational Approaches to SNP Discovery and Analysis

Jan van Oeveren and Antoine Janssen

SECTION III: SNP IDENTIFICATION AND DETECTION STRATEGIES: WHOLE GENOME SEQUENCING AND RE-SEQUENCING

5. Next-Generation Sequencing Methods: Impact of Sequencing Accuracy on SNP Discovery.

Eugene Y. Chan

6. Scanning Probe and Nanopore DNA Sequencing: Core Techniques and Possibilities. John Lund and Babak A. Parviz

7. Pyrosequencing for SNP Genotyping Jose Luis Royo and Jose Jorge Galán

SECTION IV: PRESCREENING (MELTING BASED) METHODS FOR SNP DISCOVERY AND ANALYSIS

8. Single Nucleotide Polymorphism Screening with Denaturing Gradient Gel Electrophoresis.

Leslie A. Knapp

9. Temporal Temperature Gradient Electrophoresis for Detection of Single Nucleotide Polymorphisms.

Bethan M. Jones and Leslie A. Knapp

SECTION V: PRESCREENING (CONFORMATION BASED) METHODS FOR SNP DISCOVERY AND ANALYSIS

10. Zn(II)-Cyclen Polyacrylamide Gel Electrophoresis for SNP Detection Emiko Kinoshita-Kikuta, Eiji Kinoshita, and Tohru Koike

11. Phosphate-Affinity Polyacrylamide Gel Electrophoresis for SNP Genotyping

Eiji Kinoshita, Emiko Kinoshita-Kikuta, and Tohru Koike

12. Estimation of SNP Allele Frequencies by SSCP Analysis of Pooled DNA.

Tomoko Tahira, Yoji Kukita, Koichiro Higasa, Yuko Okazaki, Aki Yoshinaga, and Kenshi Hayashi

13. Phenylethynylpyrene Excimer Forming Hybridization Probes for Fluorescence SNP Detection..

Igor A. Prokhorenko, Irina V. Astakhova, Kuvat T. Momynaliev, Timofei S. Zatsepin, and Vladimir A. Korshun

14

. The Chemical Cleavage of Mismatch for the Detection of Mutations in Long DNA Fragments

Tania Tabone, Georgina Sallmann, and Richard G. H. Cotton

15. Mismatch Oxidation Assay: Detection of DNA Mutations Using a Standard UV/Vis Microplate Reader..

Tania Tabone, Georgina Sallmann, and Richard G. H. Cotton

SECTION VI: TOWARDS HIGH-THROUGHPUT METHODS OF SNP GENOTYPING

16. High-Throughput Methods for SNP Genotyping Chunming Ding and Shengnan Jin

17. High-Throughput SNP Genotyping: Combining Tag SNPs and Molecular Beacons..

Luis B. Barreiro, Ricardo Henriques, and Musa M. Mhlanga

18. SNP Genotyping by the 5′-Nuclease Reaction: Advances in High-Throughput Genotyping with Nonmodel Organisms.

James E. Seeb, Carita E. Pascal, Ramesh Ramakrishnan, and Lisa W. Seeb

19. The TaqMan Method for SNP Genotyping Gong-Qing Shen, Kalil G. Abdullah, and Qing Kenneth Wang

20. Qualitative and Quantitative Genotyping Using Single Base Primer Extension Coupled with Matrix-Assisted Laser Desorption/Ionization

Time-of-Flight Mass Spectrometry (MassARRAY)..

Paul Oeth, Guy del Mistro, George Marnellos, Tao Shi, and Dirk van den Boom

21. SNP Detection Using Trityl Mass Tags

Klara R. Birikh, Pablo L. Bernad, Vadim V. Shmanai, Andrei D. Malakhov, Mikhail S. Shchepinov, and Vladimir A. Korshun

22. Putting the Invader Assay to Work: Laboratory Application and Data Management.

Yi Zhang, Edward Smith, and Michael Olivier

23. SNP Genotyping Using Multiplex Single Base Primer Extension Assays Daniele Podini and Peter M. Vallone

24. High-Throughput SNP Detection Based on PCR Amplification on Magnetic Nanoparticles Using Dual-Color Hybridization.

Nongyue He, Song Li, and Hongna Liu

Conte

SECTION VII: OTHER METHODS

25. Restriction Enzyme Analysis of PCR Products

Masao Ota, Hideki Asamura, Takahito Oki, and Masaharu Sada

26. Allele-Specific PCR in SNP Genotyping..

Muriel Gaudet, Anna-Giulia Fara, Isacco Beritognolo, and Maurizio Sabatti

27. Modified Multiple Primer Extension Method.

Toshihide Yanagawa and Hisashi Koga

28. Detection of SNP by the Isothermal Smart Amplification Method

Alexander Lezhava and Yoshihide Hayashizaki

Subject Index

People also search for:

    
single nucleotide polymorphisms are best described as
    
single nucleotide polymorphism function
    
single nucleotide polymorphism example
    
single nucleotide polymorphism explained
    
single nucleotide polymorphism types

Tags: Barkur Shastry, Anton Komar, Single Nucleotide, Polymorphisms Methods