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Single Nucleotide Polymorphisms Methods and Protocols 1st Edition by Pui Yan Kwok ISBN 0896039684 978-0896039681

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Single Nucleotide Polymorphisms Methods and Protocols 1st Edition Pui-Yan Kwok Digital Instant Download

Author(s): Pui-Yan Kwok
ISBN(s): 9780896039681, 0896039684
Edition: 1
File Details: PDF, 4.51 MB
Year: 2002
Language: english
SKU: EB-1618534 Category: Tag:

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Product details: 

ISBN 10: 0896039684 

ISBN 13: 978-0896039681

Author: Pui-Yan Kwok 

Single nucleotide polymorphisms (SNPs) have become the markers of choice in elucidating the relationship between DNA sequence variation and susceptibility to disease and have clearly become the focus of the next phase of the human genome project. In Single Nucleotide Polymorphisms: Methods and Protocols, Pui-Yan Kwok, MD, PhD, has assembled a collection of robust techniques for the difficult process of SNP discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection. Described in step-by-step detail by their inventors, each method provides extensive notes on the technical steps critical for experimental success, time-saving techniques, and tips on avoiding pitfalls.
Comprehensive and authoritative, Single Nucleotide Polymorphisms: Methods and Protocols provides in a readily reproducible format all the major SNP discovery and genotyping techniques in use today, whether for using DNA diagnostics to identify a pathogen, for studying the genetic basis of human disease, or for molecular breeding programs in agriculture.

Table of contents: 

1. SNPs: Why Do We Care? — Lisa D. Brooks
2. Denaturing High-Performance Liquid Chromatography — Andreas Premstaller, Peter J. Oefner
3. SNP Detection and Allele Frequency Determination by SSCP — Tomoko Tahira, Akari Suzuki, Yoji Kukita, Kenshi Hayashi
4. Conformation-Sensitive Gel Electrophoresis — Arupa Ganguly
5. Detection of Mutations in DNA by Solid-Phase Chemical Cleavage Method — Chinh T. Bui, Jeffrey J. Babon, Andreana Lambrinakos, Richard G. H. Cotton
6. SNP Discovery by Direct DNA Sequencing — Pui-Yan Kwok, Shenghui Duan
7. Computational SNP Discovery in DNA Sequence Data — Gabor T. Marth
8. Genotyping SNPs With Molecular Beacons — Salvatore A. E. Marras, Fred Russell Kramer, Sanjay Tyagi
9. SNP Genotyping by the 5′-Nuclease Reaction — Kenneth J. Livak
10. Genotyping SNPs by Minisequencing Primer Extension Using Oligonucleotide Microarrays — Katarina Lindroos, Ulrika Liljedahl, Ann-Christine Syvänen
11. Quantitative Analysis of SNPs in Pooled DNA Samples by Solid-Phase Minisequencing — Charlotta Olsson, Ulrika Liljedahl, Ann-Christine Syvänen
12. Homogeneous Primer Extension Assay With Fluorescence Polarization Detection — Tony M. Hsu, Pui-Yan Kwok
13. Pyrosequencing for SNP Genotyping — Mostafa Ronaghi
14. Homogeneous Allele-Specific PCR in SNP Genotyping — Søren Germer, Russell Higuchi
15. Oligonucleotide Ligation Assay — Jonas Jarvius, Mats Nilsson, Ulf Landegren
16. Invader Assay for SNP Genotyping — Victor Lyamichev, Bruce Neri
17. MALDI-TOF Mass Spectrometry-Based SNP Genotyping — Niels Storm, Brigitte Darnhofer-Patel

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Tags: Pui Yan Kwok, Single Nucleo, tide Polymorphisms, Methods and Protocols