Signal Transduction Protocols 2nd Editoin Methods in Molecular Biology Vol 284 2nd Edition by Robert Dickson, Michael Mendenhall ISBN 1617374482 978-1617374487

Signal Transduction Protocols 2nd Editoin Methods in Molecular Biology Vol 284 2nd Edition by Robert C. Dickson, Michael D. Mendenhall   – Ebook PDF Instant Download/Delivery: 1617374482,  978-1617374487
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Product details: 

ISBN 10: 1617374482 

ISBN 13:  978-1617374487

Author: Robert C. Dickson, Michael D. Mendenhall 

In 1995, Signal Transduction Protocols, edited by David A. Kendall and Stephen J. Hill, was published in the Methods in Molecular Biology series. This second edition represents an update to that previous work with an emp- sis on new methodologies that have developed in the last few years. The goal, then and now, is to provide procedures written by experts with first-hand ex- rience in a detail that goes far beyond what is generally encountered in the “methods” section of most journals and thus actually permits a particular p- cedure to be replicated. In addition, we have had as a secondary goal the id- tification of protocols for the assay of general classes of signal transduction components that, ideally, can be adapted to the assay of any member of that class. The ability to do this has resulted in large part from the use of affini- based assays, the ease with which specific proteins can be specifically tagged, and an explosion in the availability of highly specific antibodies from comm- cial sources, especially antibodies raised against signaling proteins of human origin. The number of available approaches is, fortunately for those working in signaling research, far too great to fit within the confines of this volume, so hard choices as to what to include had to be made.

Table of contents: 

1 Making Protein Immunoprecipitates
2 Signal Transduction Inhibitors in Cellular Function
3 Two-Dimensional Gel Electrophoresis for the Identification of Signaling Targets
4 A High-Throughput Mammalian Cell-Based Transient Transfection Assay
5 Determining Protein Half-Lives
6 Assaying Protein Kinase Activity
7 Comparative Phosphorylation Site Mapping From Gel-Derived Proteins Using a Multidimensional ES/MS-Based Approach
8 Studies of Calmodulin-Dependent Regulation
9 Measurement of Protein-DNA Interactions In Vivo by Chromatin Immunoprecipitation
10 Characterization of Protein-DNA Association In Vivo by Chromatin Immunoprecipitation
11 Nonradioactive Methods for Detecting Activation of Ras-Related Small G Proteins
12 Nucleocytoplasmic Glycosylation, O-GlcNAc
13 Techniques in Protein Methylation
14 Assaying Lipid Phosphate Phosphatase Activities
15 Assaying Phosphoinositide Phosphatases
16 Assaying Phospholipase A2 Activity
17 Measurement and Immunofluorescence of Cellular Phosphoinositides
18 Measuring Dynamic Changes in cAMP Using Fluorescence Resonance Energy Transfer
19 In Vivo Detection of Protein-Protein Interaction in Plant Cells Using BRET

20 Revealing Protein Dynamics by Photobleaching Techniques
21 Assaying Cytochrome c Translocation During Apoptosis

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Robert Dickson,Michael Mendenhall,Signal Transduction