RNA Interference Techniques 1st Edition by Yu Shen ISBN 9781617791147 1617791148
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ISBN 10: 1617791148
ISBN 13: 9781617791147
Author: Yu Shen
RNA Interference Techniques, edited by Scott Q. Harper, is a specialized volume in the Neuromethods series, published by Humana Press in 2011. This book provides a comprehensive collection of protocols and methodologies for employing RNA interference (RNAi) in gene silencing, particularly within neural tissues. It serves as a valuable resource for researchers aiming to manipulate gene expression in the nervous system and other biological contexts
Table of contents:
1. Designing Hairpin-Based RNAi Shuttles.
Yu Shen
2. Rapid Cloning and Validation of MicroRNA Shuttle Vectors: A Practical Guide
Ryan L. Boudreau, Sara E. Garwick-Coppens, Jian Liu, Lindsay M. Wallace, and Scott Q. Harper
3. The Use of Small Noncoding RNAs to Silence Transcription in Human Cells Kevin V. Morris Delivery Strategies for RNAi to the Nervous System.
4 Kevin D. Foust and Brian K. Kaspar
5. Cloning Small RNAs. Eric J. Devor and Lingyan Huang
6. Profiling the miRNome: Detecting Global miRNA Expression Levels with DNA Microarrays
Peter White
7. High-Throughput Profiling of Mature MicroRNA by Real-Time PCR
Jinmai Jiang, Eun Joo Lee, Melissa G. Piper, Clay B. Marsh, and Thomas D. Schmittgen
8. In Situ Detection of MicroRNAs in Paraffin-Embedded, Formalin-Fixed Tissues:
Different Methodologies and Co-localization with Possible Targets Gerard J. Nuovo, Patrick Nana-Sinkam, Melissa Crawford, and Thomas D. Schmittgen
9. Tracing of siRNAs Inside Cells by FRET Imaging.
Markus Hirsch, Il-Han Kim, Anne Järve, Roger Fischer, Michael F. Trendelenburg, Ulrich Massing, Karl Rohr, and Mark Helm
10. Application of RIP-Chip for the Identification of miRNA Targets. Lu Ping Tan, Anke van den Berg, and Joost L. Kluiver
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Tags: Yu Shen, RNA Interference Techniques