RNA Interference From Biology to Clinical Applications 1st Edition by Abubaker Sidahmed, Bruce Wilkie, Wei Ping Min, Thomas Ichim ISBN 9781607615880 1607615886

RNA Interference From Biology to Clinical Applications 1st Edition by  Abubaker M. E. Sidahmed, Bruce Wilkie (auth.), Wei-Ping Min, Thomas Ichim  – Ebook PDF Instant Download/Delivery: 9781607615880, 1607615886 
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Product details: 

ISBN 10:  1607615886 

ISBN 13: 9781607615880

Author: Abubaker M. E. Sidahmed, Bruce Wilkie (auth.), Wei-Ping Min, Thomas Ichim 

From the early days when RNA interference was a strange artifact in worms to the 2006 Noble Prize received by Fire and Mello and the current clinical trials, the field of RNA interference has grown at a breakneck pace. In RNA Interference: From Biology to Clinical Applications, expert contributors provide an overview of the most current science and protocols that span the biological disciplines from detailed nucleic acid chemistry, to pharmacology, to the manipulation of signal transduction pathways. Divided into three distinct sections, this volume delves into the physiology of RNA interference, RNA interference in the laboratory and siRNA delivery, and preclinical and clinical issues associated with the use of RNAi-inducing agents as drugs in order to stimulate new questions and offer the tools necessary to start addressing those questions. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Table of contents: 

1. Physiology of RNA Interference

Section I: Mechanisms and Molecular Basis
2. Endogenous Antiviral Mechanisms of RNA Interference: A Comparative Biology Perspective – Abubaker M. E. Sidahmed, Bruce Wilkie
3. Monitoring Innate Immune Recruitment by siRNAs in Mammalian Cells – Michael P. Gantier, Bryan R. G. Williams
4. Current Knowledge of MicroRNAs and Noncoding RNAs in Virus-Infected Cells – Dominique L. Ouellet, Patrick Provost
5. Allele-Specific Silencing by RNA Interference – Hirohiko Hohjoh
6. Computational siRNA Design Considering Alternative Splicing – Young J. Kim
7. Bioinformatic Approaches to siRNA Selection and Optimization – Pirkko Muhonen, Harry Holthofer
8. Optimized Gene Silencing by Co-expression of Multiple shRNAs in a Single Vector – Yasuhito Ishigaki, Akihiro Nagao, Tsukasa Matsunaga
9. Strategies in Designing Multigene Expression Units to Downregulate HIV-1 – Jane Zhang, John J. Rossi
10. Designing Optimal siRNA Based on Target Site Accessibility – Ivo L. Hofacker, Hakim Tafer
11. Chemical Synthesis of 2′-O-Alkylated siRNAs – Joachim W. Engels, Dalibor Odadzic, Romualdas Smicius, Jens Haas

Section II: RNA Interference in the Laboratory and Delivery Methods
12. RNA Interference in the Laboratory and siRNA Delivery
13. siRNA Specific Delivery System for Targeting Dendritic Cells – Xiufen Zheng, Costin Vladau, Aminah Shunner, Wei-Ping Min
14. Hydrodynamic Delivery Protocols – Piotr G. Rychahou, B. Mark Evers
15. New Methods for Reverse Transfection with siRNA from a Solid Surface – Satoshi Fujita, Kota Takano, Eiji Ota, Takuma Sano, Tomohiro Yoshikawa, Masato Miyake et al.
16. Nonviral siRNA Delivery for Gene Silencing in Neurodegenerative Diseases – Satya Prakash, Meenakshi Malhotra, Venkatesh Rengaswamy
17. Using siRNA to Uncover Novel Oncogenic Signaling Pathways – Jin-Mei Lai, Chi-Ying F. Huang, Chang-Han Chen
18. Biodegradable Polymer-Mediated sh/siRNA Delivery for Cancer Studies – Dhananjay J. Jere, Chong-Su Cho
19. Cellular siRNA Delivery Using TatU1A and Photo-Induced RNA Interference
20. Polyethylenimine (PEI)/siRNA-Mediated Gene Knockdown In Vitro and In Vivo – Sabrina Höbel, Achim Aigner
21. Transfection of siRNAs in Multiple Myeloma Cell Lines – Jose L. R. Brito, Nicola Brown, Gareth J. Morgan

Section III: Clinical Implementation
22. A New Approach for Therapeutic Use by RNA Interference in the Brain – Yukio Akaneya
23. Inhibitory RNA Molecules in Immunotherapy for Cancer – Chih-Ping Mao, T.-C. Wu
24. Preventing Tissue Injury Using siRNA – Zhu-Xu Zhang, Marianne E. Beduhn, Xiufen Zheng, Wei-Ping Min, Anthony M. Jevnikar
25. Preventing Immune Rejection Through Gene Silencing – Xusheng Zhang, Mu Li, Wei-Ping Min
26. Topical Application of siRNA Targeting Cutaneous Dendritic Cells in Allergic Skin Disease – Miyuki Azuma, Patcharee Ritprajak, Masaaki Hashiguchi
27. Direct Application of siRNA for In Vivo Pain Research – Philippe Sarret, Louis Doré-Savard, Nicolas Beaudet
28. A Potential Therapeutic for Pandemic Influenza Using RNA Interference – Shaguna Seth, Michael V. Templin, Gregory Severson, Oleksandr Baturevych
29. Evaluation of Targets for Ovarian Cancer Gene Silencing Therapy: In Vitro and In Vivo Approaches

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Tags: Abubaker Sidahmed, Bruce Wilkie, Wei Ping Min, Thomas Ichim, RNA Interference, From Biology