Proteomic profiling methods and protocols Second Edition by Anton Posch ISBN 9781071611852 1071611852

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ISBN 10:  1071611852

ISBN 13:  9781071611852

Author:  Anton Posch

This detailed new edition presents the latest developments of the main pillars of protein analysis, namely sample preparation, separation, and characterization. Core areas in this volume are protocols for the analysis of post-translational modifications and protein interaction partners, followed by sophisticated procedures to enrich for extracellular vesicles and organelles, along with several types of protein immuno-assays complemented by various methods for the characterization of antibodies and host-cell protein analysis. Last but not least, a few standard sample preparation protocols and recent advances concerning immuno-chemical detection of proteins are included as well. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Proteomic Profiling: Methods and Protocols, Second Edition serves as an ideal reference for students of biochemistry, biomedicine, biology, and genomics and will be an invaluable source for the experienced, practicing scientist as well.

Table of contents: 

1. Chromatin Immunoprecipitation (ChIP) to Study DNA–Protein Interactions
   Eliza C. Small, Danielle N. Maryanski, Keli L. Rodriguez, Kevin J. Harvey, Michael-C. Keogh, Andrea L. Johnstone

2. Profiling Protein–DNA Interactions by Chromatin Immunoprecipitation in Arabidopsis
   Hendry Susila, Zeeshan Nasim, Suhyun Jin, Geummin Youn, Hyewon Jeong, Ji-Yul Jung et al.

3. Biotin Proximity Labeling for Protein–Protein Interaction Discovery: The BioID Method
   Jeff E. Habel

4. Studying OTUD6B-OTUB1 Protein–Protein Interaction by Low-Throughput GFP-Trap Assays and High-Throughput AlphaScreen Assays
   Elisabeth Weber, Kenji Schorpp, Kamyar Hadian

5. Thermal Shift Assay for Exploring Interactions Between Fatty Acid–Binding Protein and Inhibitors
   Jiaqing Hao

6. Isolation and Purification of Mitochondria from Cell Culture for Proteomic Analyses
   Yaschar Kabiri, Christine von Toerne, Adriana Fontes, Percy A. Knolle, Hans Zischka

7. Investigating the Adipose Tissue Secretome: A Protocol to Generate High-Quality Samples Appropriate for Comprehensive Proteomic Profiling
   Sonja Hartwig, Jorg Kotzka, Stefan Lehr

8. Methods for Proteomics-Based Analysis of the Human Muscle Secretome Using an In Vitro Exercise Model
   Cora Weigert, Sonja Hartwig, Stefan Lehr

9. Western Blotting Using In-Gel Protein Labeling as a Normalization Control: Advantages of Stain-Free Technology
   Rômulo Leão Silva Neris, Andrea Marie Chua Dobles, Aldrin V. Gomes

10. Technical Considerations for Contemporary Western Blot Techniques
    Kenneth Oh

11. Simple Western: Bringing the Western Blot into the Twenty-First Century
    Carsten Lück, Charles Haitjema, Christopher Heger

12. Development of Peptide Ligands for Targeted Capture of Host Cell Proteins from Cell Culture Production Harvests
    R. Ashton Lavoie, Taufika Islam Williams, R. Kevin Blackburn, Ruben G. Carbonell, Stefano Menegatti

13. Sample Preparation of Secreted Mammalian Host Cell Proteins and Their Characterization by Two-Dimensional Electrophoresis and Western Blotting
    Anton Posch, Franziska Kollmann, Thomas Berkelman, Elizabeth Dreskin

14. Quantitative Proteomic Analysis Using Formalin-Fixed, Paraffin-Embedded (FFPE) Human Cardiac Tissue
    Omid Azimzadeh, Michael J. Atkinson, Soile Tapio

15. Chloroplast Isolation and Enrichment of Low-Abundance Proteins by Affinity Chromatography for Identification in Complex Proteomes
    Roman G. Bayer, Simon Stael, Markus Teige

16. Principles of Protein Labeling Techniques
    Christian Obermaier, Anja Griebel, Reiner Westermeier

17. Mechanical/Physical Methods of Cell Disruption and Tissue Homogenization
    Stanley Goldberg

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