Protein Purification Principles High Resolution Methods and Applications Methods of Biochemical Analysis 3rd Edition by Jan Christer Janson ISBN 978-0471746614 0471746614

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Product details: 

ISBN 10:  0471746614

ISBN 13: 978-0471746614

Author: Jan-Christer Janson

The authoritative guide on protein purification―now completely updated and revised

Since the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters rewritten from scratch.

Leading experts in the field cover all major biochemical separation methods for proteins in use today, providing professionals in biochemistry, organic chemistry, and analytical chemistry with quick access to the latest techniques. Entirely new or thoroughly revised content includes:

High-resolution reversed-phase liquid chromatography

Electrophoresis in gels

Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients

Affinity ligands from chemical and biological combinatorial libraries

Membrane separations

Refolding of inclusion body proteins from E. coli

Purification of PEGylated proteins

High throughput screening techniques in protein purification

The history of protein chromatography

Table of contents: 

Part I: Introduction
An Introduction to Protein Purification
Bo Ersson, Lars Rydén, and Jan-Christer Janson

Part II: Chromatography
Fundamentals of Chromatography
Jan-Christer Janson and Jan Åke Jönsson

Size Exclusion Chromatography (Gel Filtration)
Lars Hagel

Ion Exchange Chromatography Techniques
Evert Karlsson and Irwin Hirsh

High-Resolution Reversed-Phase Chromatography of Proteins
Sylvia Winkel Pettersson

Hydrophobic Interaction Chromatography
Kjell-Ove Eriksson and Makonnen Belew

Immobilized Metal Ion Affinity Chromatography (IMAC)
Lennart Kågedal

Covalent Chromatography Methods
Francisco Batista-Viera, Lars Rydén, and Jan Carlsson

Affinity Chromatography Principles and Applications
Francisco Batista-Viera, Jan-Christer Janson, and Jan Carlsson

Affinity Ligands Derived from Chemical Combinatorial Libraries
Enrique Carredano and Herbert Baumann

Affinity Ligands Derived from Biological Combinatorial Libraries
Per-Åke Nygren

Part III: Other Separation Methods and Related Techniques
Membrane-Based Separation Technologies
Joachim K. Walter, Zuwei Jin, Maik W. Jornitz, and Uwe Gottschalk

Refolding of Inclusion Body Proteins Expressed in E. coli
Zhiguo Su, Diannan Lu, and Zheng Liu

Purification of PEGylated Proteins
Conan J. Fee and James M. Van Alstine

Part IV: Electrophoresis
Gel Electrophoresis Techniques
Reiner Westermeier

Isoelectric Focusing in Gel Slabs, Capillaries, and Immobilized pH Gradients
Pier Giorgio Righetti, Elisa Fasoli, and Sabina Carla Righetti

Two-Dimensional Electrophoresis in Proteomic Analysis
Reiner Westermeier and Angelika Görg

Protein Elution and Blotting Techniques
Reiner Westermeier

Capillary Electrophoretic Separation Methods
Wolfgang Thormann

Part V: Separation Method Optimization
High-Throughput Screening Strategies in Protein Purification
Karol M. Lacki and Eggert Brekkan

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