Plant Pathology Techniques and Protocols 2nd 2015 Edition by Christophe Lacomme- Ebook PDF Instant Download/Delivery: 978-1493926190, 1493926195
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Product details:
ISBN 10: 1493926195
ISBN 13: 978-1493926190
Author: Christophe Lacomme
The second edition of Plant Pathology: Techniques and Protocols covers diagnostic methods that are currently used in laboratories for a broad range of plant species and matrixes. These include serological and molecular methods that have one or more of the following characteristics: suitability for high-throughput testing, detection of a group of pathogens or of sometimes uncharacterized pathogens, detection and identification of specific pathogens, and high sensitivity. This volume discusses qualitative and quantitative tests, as well as recently developed diagnostic methods. It also provides background information on many pathogens, which are either endemic, non-endemic, or emerging and with different lifecycles that cause diseases of significant importance in a wide variety of hosts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, background information on pathogens and the disease caused, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Informative and cutting-edge, Plant Pathology: Techniques and Protocols, Second Edition is the perfect book for plant pathologists and molecular biologists who will use this information to test out the latest research in their laboratories.
Table of contents:
1 Detection of the Bacterial Potato Pathogens Pectobacterium and Dickeya spp. Using Conventional and Real-Time PCR Sonia N. Humphris, Greig Cahill, John G. Elphinstone, Rachel Kelly, Neil M. Parkinson, Leighton Pritchard, Ian K. Toth, and Gerry S. Saddler
2 Detection and Identification of Phoma Pathogens of Potato Denise A’Hara
3 Diagnosis of Ramularia collo-cygni and Rhynchosporium spp. in Barley Neil Havis, James Fountaine, Kalina Gorniak, Linda Paterson, and Jeanette Taylor
4 A Real-Time Multiplex PCR Assay Used in the Identification of Closely Related Fungal Pathogens at the Species Level Dominie G. Wright
5 Diagnostics of Tree Diseases Caused by Phytophthora austrocedri Species….. Vincent Mulholland, Matthew Elliot, and Sarah Green
6 Real-Time LAMP for Chalara fraxinea Diagnosis. Jenny Tomlinson and Neil Boonham
7 Loop-Mediated Isothermal Amplification Procedure (LAMP) for Detection of the Potato Zebra Chip Pathogen “Candidatus Liberibacter solanacearum” Aravind Ravindran, Julien Lévy, Elizabeth Pierson, and Dennis C. Gross
8 Loop-Mediated Isothermal Amplification (LAMP) for Detection of Phytoplasmas in the Field. Matt Dickinson
9 Diagnosis of Phytoplasmas by Real-Time PCR Using Locked Nucleic Acid (LNA) Probes Sabrina Palmano, Vincent Mulholland, David Kenyon, Gerry Saddler, and Colin Jeffries
10 Q-Bank Phytoplasma: A DNA Barcoding Tool for Phytoplasma Identification Nicoletta Contaldo, Samanta Paltrinieri, Olga Makarova, Assunta Bertaccini, and Mogens Nicolaisen
11 High-Throughput Diagnosis of Potato Cyst Nematodes in Soil Samples Alex Reid, Fiona Evans, Vincent Mulholland, Yvonne Cole, and Jon Pickup
12 Detection of Nepovirus Vector and Nonvector Xiphinema Species in Grapevine C. Van Ghelder, A. Reid, D. Kenyon, and D. Esmenjaud
13 Molecular and Serological Methods for the Diagnosis of Viruses in Potato Tubers.. Christophe Lacomme, Ross Holmes, and Fiona Evans
14 Immunocapture-Multiplex RT-PCR for the Simultaneous Detection and Identification of Plant Viruses and Their Strains: Study Case, Potato Virus Y (PVY).. Mohamad Chikh-Ali and Alexander V. Karasev
15 SNaPshot and CE-SSCP: Two Simple and Cost-Effective Methods to Reveal Genetic Variability Within a Virus Species. Agnès Delaunay, Sylvie Dallot, Denis Filloux, Virginie Dupиу, Philippe Roumagnac, and Emmanuel Jacquot
16 Detection and Characterization of Viral Species/Subspecies Using Isothermal Recombinase Polymerase Amplification (RPA) Assays Laurent Glais and Emmanuel Jacquot
17 Virus Testing by PCR and RT-PCR Amplification in Berry Fruit…. Stuart MacFarlane, Wendy McGavin, and Ioannis Tzanetakis
18 Metagenomics Approaches Based on Virion-Associated Nucleic Acids (VANA): An Innovative Tool for Assessing Without A Priori Viral Diversity of Plants. Denis Filloux, Sylvie Dallot, Agnès Delaunay, Serge Galzi, Emmanuel Jacquot, and Philippe Roumagnac
19 Detection of Potato spindle tuber viroid and Other Related Viroids by a DIG Labelled RNA Probe. Wendy A. Monger and Colin Jeffries
20 Microarray Platform for the Detection of a Range of Plant Viruses and Viroids. Ian Adams, Catherine Harrison, Jenny Tomlinson, and Neil Boonham
21 Multiplex Detection of Plant Pathogens Through the Luminex Magplex Bead System. René A.A.
22 Next-Generation Sequencing of Elite Berry Germplasm and Data Analysis Using a Bioinformatics Pipeline for Virus Detection and Discovery.. Thien Ho, Robert R. Martin, and Ioannis E. Tzanetakis
23 Metagenomic Next-Generation Sequencing of Viruses Infecting Grapevines. Johan T. Burger and Hans J. Maree
24 Droplet Digital PCR for Absolute Quantification of Pathogens. Ion Gutiérrez-Aguirre, Nejc Rački, Tanja Dreo, and Maja Ravnikar
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