Sale!

Plant Cell Expansion Methods and Protocols 1st Edition by José Estevez 1493919016 9781493919017

Original price was: $50.00.Current price is: $35.00.

Plant Cell Expansion Methods and Protocols 1st Edition José M. Estevez (Eds.) Digital Instant Download

Author(s): José M. Estevez (eds.)
ISBN(s): 9781493919017, 1493919016
Edition: 1
File Details: PDF, 8.42 MB
Year: 2015
Language: english
SKU: EB-4947352 Category: Tag:

Plant Cell Expansion Methods and Protocols 1st Edition by José M. Estevez – Ebook PDF Instant Download/Delivery: 1493919016, 978-1493919017
Full dowload Plant Cell Expansion Methods and Protocols 1st Edition after payment

 

Product details:

ISBN 10:         1493919016
ISBN 13: 978-1493919017
Author:          José M. Estevez

This volume covers broad aspects of cell expansion in three different cell types: root hairs, pollen tubes, and hypocothyl cells. Chapters focus on the cutting-edge methods to study in detail several complex aspects of cell expansion such as secretion, endocytosis and recycling, cellular signaling and trafficking, and protein and polysaccharides cell wall biosynthesis in real time during cell expansion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Cell Expansion: Methods and Protocols is an essential reference book for plant scientist, molecular, and cell biologist as well as plant biochemists​.

Plant Cell Expansion Methods and Protocols 1st Table of contents:

Part I: General Overview of Plant Cell Expansion

  1. Introduction to Plant Cell Expansion

    • Basic Concepts and Importance of Cell Expansion
    • Mechanisms of Cell Wall Growth and Expansion
    • Role of Hormones in Cell Expansion
    • Environmental and Genetic Regulation

  2. Experimental Approaches to Study Plant Cell Expansion

    • Overview of Methodologies
    • Key Challenges in Measuring Cell Expansion
    • Ethical Considerations in Plant Research

Part II: Techniques for Measuring and Analyzing Plant Cell Expansion

  1. Measurement of Plant Cell Growth

    • Microscopy-Based Methods for Cell Expansion
    • Use of Atomic Force Microscopy (AFM)
    • Image Analysis and Quantification Techniques

  2. Assessment of Cell Wall Properties and Mechanics

    • Mechanical Strength and Elasticity of Cell Walls
    • Cell Wall Biochemistry and Composition Analysis
    • Use of Biophysical Techniques to Study Cell Wall Changes

  3. Tracking Cell Expansion Using Molecular Tools

    • Reporter Gene Technology
    • Gene Expression Profiling of Expanding Cells
    • Fluorescence-Based Techniques for Cell Tracking

Part III: Molecular and Genetic Tools for Studying Cell Expansion

  1. Role of Cell Wall Modifying Enzymes in Expansion

    • Pectinases, Xylanases, and Cellulases in Plant Growth
    • Gene Knockout Approaches to Study Enzyme Functions
    • Functional Genomics for Understanding Cell Wall Modification

  2. Hormonal Regulation of Cell Expansion

    • Role of Auxin, Gibberellin, and Cytokinin
    • Hormone-Responsive Gene Expression Studies
    • Methods for Manipulating Hormonal Pathways in Plants

  3. Gene Editing and Manipulation for Studying Cell Expansion

    • CRISPR/Cas9 Applications in Plant Cell Expansion Research
    • Transgenic Plant Models
    • RNA Interference (RNAi) and Overexpression Studies

Part IV: Specific Protocols for Studying Plant Cell Expansion

  1. Protocol for Measuring Cell Expansion in Arabidopsis

    • Growth Conditions and Preparation of Plant Samples
    • Protocol for Measuring Cell Size and Shape
    • Data Collection and Analysis Techniques

  2. Protocol for Quantifying Cell Wall Expansion in Tobacco Cells

    • Sample Preparation and Staining Techniques
    • Mechanical Testing for Cell Wall Properties
    • Using Microscopy to Visualize Cell Wall Dynamics

  3. Protocols for Hormonal Treatments and Monitoring Cell Growth

    • Application of Exogenous Hormones to Induce Expansion
    • Measuring Hormonal Effects on Cell Growth
    • Case Studies in Hormonal Control of Expansion

  4. Protocol for Gene Expression Analysis in Expanding Cells

    • RNA Extraction and Quantification Techniques
    • PCR and RT-PCR for Gene Expression Analysis
    • Northern Blotting and In Situ Hybridization

Part V: Advanced Topics in Plant Cell Expansion

  1. Intercellular Signaling in Cell Expansion

    • Role of Calcium Signaling and Phosphorylation Pathways
    • Crosstalk Between Hormonal and Mechanical Signals
    • Techniques for Investigating Signal Transduction in Expanding Cells

  2. Cell Expansion in Response to Stress

    • Effects of Abiotic Stress on Cell Growth and Expansion
    • Protocols for Studying Osmotic Stress and Heat Shock Responses
    • Mechanisms of Stress-Induced Cell Wall Modification

  3. Cell Expansion in Plant Development and Growth

    • Role of Cell Expansion in Organ Development
    • Expansion in Root and Shoot Systems
    • Cell Expansion During Fruit and Seed Development

Part VI: Future Directions and Challenges in Plant Cell Expansion Research

  1. Emerging Techniques and Technologies in Plant Cell Expansion

    • Advanced Imaging Techniques (e.g., Super-Resolution Microscopy)
    • In Vivo Imaging of Plant Growth Dynamics
    • Use of 3D Culture Systems and Microfluidics

  2. Challenges and Prospects for Translational Research

    • Scaling from Model Systems to Crop Plants
    • Engineering Plants for Improved Growth and Yield
    • The Role of Synthetic Biology in Plant Cell Expansion

People also search for Plant Cell Expansion Methods and Protocols 1st:

plant cell expansion
a plant cell membrane
do plant cells differentiate
do plant cells have extracellular matrix
in plant cells the middle lamella

Tags:

José M. Estevez,Plant,Cell,Expansion,Methods,Protocols 1st