Photoproteins in Bioanalysis 1st Edition by Sylvia Daunert, Sapna Deo ISBN 3527310166 978-3527310166

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Product details: 

ISBN 10:  3527310166 

ISBN 13: 978-3527310166

Author:  Sylvia Daunert, Sapna K. Deo

The use of light-emitting proteins for the detection of biomolecules provides fast and sensitive methods which overcome the disadvantages of radioactive labels and the high cost of fluorescent dyes.
This reference work summarizes modern advanced techniques and their applications and includes practical examples of assays based on photoproteins. The book presents contemporary key topics like luminescent marine organisms, DNA probes, reporter gene assays and photoproteins, ratiometric sensing, use of photoproteins for in vivo functional imaging and luminescent proteins in binding assays, to name just a few, and is complemented by recent advances in instrumentation.
Includes an introductory chapter by 2008 Chemistry Nobel laureate Osamu Shimomura.

Table of contents: 

1. Ctenophore Photoproteins

2. Pholasin (Pholas Luciferin)

3. Chaetopterus Photoprotein

4. Polynoidin

5. Symplectin

6. Luminodesmus Photoprotein

7. Ophiopsila Photoprotein

8. Basic Strategy of Extracting and Purifying Photoproteins

9. The Photoprotein Aequorin

10. Extraction and Purification of Aequorin

11. Hydrophobic Interaction Chromatography

12. Properties of Aequorin

13. Stability

14. Freeze-drying

15. Specificity to Ca²⁺

16. Luminescence of Aequorin by Substances Other Than Divalent Cations

17. Mechanism of Aequorin Luminescence and Regeneration of Aequorin

18. Structure of Aequorin

19. Luminescence Reaction

20. Regeneration

21. Inhibitors of Aequorin Luminescence

22. Recombinant Aequorin

23. Semi-synthetic Aequorins

24. e-Aequorins

25. References

26. Luminous Marine Organisms

27. Introduction

28. Non-luminous Taxa

29. Luminous Taxa

30. Nucleic Acid Hybridization Assays

31. Other Binding Assays

32. Concluding Remarks

33. References

34. Luminescent Proteins: Applications in Microfluidics and Miniaturized Analytical Systems

35. Miniaturization and Microfluidics

36. Photoproteins and Applications in Miniaturized Detection Systems

37. Green Fluorescent Protein

38. GFP in Miniaturized Microfluidic-based Assays

39. Luciferase

40. Luciferase in Miniaturized Microfluidic-based Assays

41. Aequorin

42. Aequorin in Miniaturized Microfluidic-based Assays

43. Future Perspectives

44. References

45. Advances in Instrumentation for Detecting Low-level Bioluminescence and Fluorescence

46. Introduction

47. Low Light Levels

48. Methods of Coupling the Signal to the Detector

49. Proximity Focusing

50. Microscope Objectives

51. Macro Lenses

52. Fiber Optics

53. Evaluating the Performance of an Optical System

54. Numerical Aperture

55. Transmission Efficiency

56. Magnification

57. Detector Technologies

58. Selecting the Right Detector

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Tags: Sylvia Daunert, Sapna Deo, Photoproteins in Bioanalysis