Permeability Barrier Methods and Protocols 1st Edition by Arne Naegel ISBN 978-1617791918 1617791918

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Product details: 

ISBN 10: 1617791918

ISBN 13: 978-1617791918  

Author: Arne Naegel

The significant biological subject, the permeability barrier, is incredibly diverse and vital for a vast assortment of crucial functions in the body.  In Permeability Barrier: Methods and Protocols, a variety of experienced researchers contribute techniques to study this complex system in its many forms.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Permeability Barrier: Methods and Protocols serves as an ideal guide for all scientists seeking to further our understanding of this vital area of research.

Table of contents: 

1. Computational Modeling of the Skin Barrier
2. In Vitro Human Skin Segmentation and Drug Concentration–Skin Depth Profiles
3. Transcriptional Regulation of Epidermal Barrier Formation
4. Epidermal Permeability Barrier Measurement in Mammalian Skin
5. Assessment of Permeability Barriers to Macromolecules in the Rodent Endometrium at the Onset of Implantation
6. Assessment of Intestinal Permeability in Premature Neonates by Sugar Absorption Tests
7. Analysis of Epithelial Cell Shedding and Gaps in the Intestinal Epithelium
8. Evaluation of Permeability in T84 Intestinal Epithelial Cells
9. Optimization of the Caco-2 Permeability Assay for Drug Screening
10. Modulation of Cell Contacts and Adhesion-Dependent Functions by Ouabain
11. Long-Term Live-Cell Imaging of Epithelial Dome Formation Using Novel Culture Systems
12. Measurement of Permeability in Human Retinal Epithelial Cells and Implications for Diabetic Retinopathy
13. Assessment of Epithelial Barrier Integrity in Polarized Lung Epithelial Cells
14. In Vitro Evaluation of Permeability in Differentiated Human Urothelium
15. Histological and Immunohistochemical Phenotyping of Claudin 11 Deficiency in Testis
16. In Vitro System for Studying Sertoli Cell Blood–Testis Barrier Dynamics
17. In Vitro Analysis of Endothelial Barrier Function
18. Role of Endothelial Cell–Cell Junctions in Regulating Endothelial Permeability
19. In Vitro Assessment of Endothelial Cell Permeability
20. Mechanotransduction and Barrier Regulation in Lung Microvascular Endothelial Cells
21. Role of Caveolin-1 in Pulmonary Endothelial Permeability Regulation
22. Assessment of Endothelial Permeability and Leukocyte Transmigration in Human Endothelial Monolayers
23. Evaluation of Endothelial Barrier Permeability Using In Vitro and In Vivo Models
24. Size-Selective Assessment of Inner Blood–Retina Barrier Permeability In Vitro
25. Evaluation of Brain Endothelial Barrier Permeability Using Tracers
26. Assessment of FcRn-Mediated Reverse Transcytosis Across the Blood–Brain Barrier
27. Evaluation of Vascular Permeability Induced by VEGF in Mouse Models
28. In Vivo Assessment of Glioma-Induced Vascular Permeability
29. Optical Imaging of Ischemia-Induced Blood–Brain Barrier Disruption
30. Back Matter

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Tags: Arne Naegel, Permeability Barrier, Methods and Protocols