1 Detecting and Quantifying p53 Isoforms at mRNA Level in Cell Lines and Tissues
2 Detecting p53 Isoforms at Protein Level
3 Autophagy, Senescence, and Apoptosis
4 p53 and Cell Cycle Effects After DNA Damage
5 p53 Ubiquitination and Proteasomal Degradation
6 Identification of p53 in Mitochondria
7 Identification of Novel Mutant p53 Interacting Proteins by Proteomic Analysis
8 Identification of Small Molecules Affecting p53-MDM2/MDMX Interaction by Fluorescence Polarization
9 Determine the Effect of p53 on Chemosensitivity
10 Measurement of Chemosensitivity and Growth Rate in p53 Expressing Cells
11 Mutant p53 in Cell Adhesion and Motility
12 Use of the DNA Fiber Spreading Technique to Detect the Effects of Mutant p53 on DNA Replication
13 Generation of p53-Deficient Induced Pluripotent Stem Cells from Mouse Embryo Fibroblasts
14 p53 Actions on MicroRNA Expression and Maturation Pathway
15 Isolation and Characterization of Murine Multipotent Lung Stem Cells
16 Generation of p53 Knock-down Cell Lines
17 ChIP for Identification of p53 Responsive DNA Promoters
18 ChIP-on-chip to Identify Mutant p53 Targets
19 ChIP Sequencing to Identify p53 Targets
p53 Protocols 2nd Edition by Sumitra Deb, Swati Palit Deb ISBN 9781627032360 1627032363
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ISBN 10: 1627032363
ISBN 13: 9781627032360
Author: Sumitra Deb, Swati Palit Deb
The second edition of p53 Protocols expands upon the first edition with progress in p53 research since the publication of the first edition. In p53 Protocols, Second Edition expert researchers in the field detail many of the methods that address the challenging questions of the p53 field. These methods include the identification of the target genes and binding partners of gain of function p53 mutants, methods to determine stress response, autophagy or senescence induced by p53, cell cycle analysis, analysis of different phases of DNA replication modified by p53, and generation of pluripotent stem cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, p53 Protocols, Second Edition seeks to aid scientists in the further study into p53 and other tumor suppressors.
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Tags: Sumitra Deb, Swati Palit Deb, p53 Protocols