Nucleic Acid Detection Methods and Protocols 1st Edition by Dmitry Kolpashchikov, Yulia Gerasimova ISBN 9781627035354 1627035354

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Product details: 

ISBN 10:1627035354

ISBN 13: 9781627035354

Author: Dmitry M. Kolpashchikov, Yulia V. Gerasimova

In Nucleic Acid Chemistry: Methods and Protocols, expert researches in the field detail techniques and approaches for the detection of DNA and RNA. These techniques include the recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, new application of isothermal amplification techniques, assays with visual or electric signals for point-of-care diagnostics, improvement of fluorescent in situ hybridization, and new signal amplification techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Nucleic Acid Chemistry: Methods and Protocols seeks to aid scientists in the further study of detection for DNA and RNA.

Table of contents: 

1. Front Matter

2. “Getting Blood from a Stone”: Ultrasensitive Forensic DNA Profiling of Microscopic Bio-Particles Recovered from “Touch DNA” Evidence
Erin K. Hanson, Jack Ballantyne

3. Detecting MicroRNA in Human Cancer Tissues with Fluorescence In Situ Hybridization
Zonggao Shi, Jeff J. Johnson, M. Sharon Stack

4. New Real-Time and Instantaneous Assays – Front Matter

5. MNAzyme qPCR: A Superior Tool for Multiplex qPCR
Elisa Mokany, Alison V. Todd

6. A New, Multiplex, Quantitative Real-Time Polymerase Chain Reaction System for Nucleic Acid Detection and Quantification
Fang Liang, Neetika Arora, Kang Liang Zhang, David Che Cheng Yeh, Richard Lai, Darnley Pearson et al.

7. Detection of SNP-Containing Human DNA Sequences Using a Split Sensor with a Universal Molecular Beacon Reporter
Yulia V. Gerasimova, Jack Ballantyne, Dmitry M. Kolpashchikov

8. SNP Analysis Using a Molecular Beacon-Based Operating Cooperatively (OC) Sensor
Evan M. Cornett, Dmitry M. Kolpashchikov

9. Isothermal Amplification Methods for Nucleic Acid Detection – Front Matter

10. Detection of rpoB Gene Mutations Using Helicase-Dependent Amplification
Wanyuan Ao, Robert Jenison

11. Rapid Detection of Brucella spp. Using Loop-Mediated Isothermal Amplification (LAMP)
Shouyi Chen, Xunde Li, Juntao Li, Edward R. Atwill

12. Loop-Mediated Isothermal Amplification Method for a Differential Identification of Human Taenia Tapeworms
Yasuhito Sako, Agathe Nkouawa, Tetsuya Yanagida, Akira Ito

13. Detection of Mutation by Allele-Specific Loop-Mediated Isothermal Amplification (AS-LAMP)
Hiroka Aonuma, Athanase Badolo, Kiyoshi Okado, Hirotaka Kanuka

14. Signal Amplification Approach – Front Matter

15. DNA Detection by Cascade Enzymatic Signal Amplification
Bingjie Zou, Yinjiao Ma, Guohua Zhou

16. Non-fluorescent Detection Formats – Front Matter

17. Visual DNA Detection and SNP Genotyping Using Asymmetric PCR and Split DNA Enzymes
Jia Ling Neo, Mahesh Uttamchandani

18. Detection of Single-Stranded Nucleic Acids via Colorimetric Means, Using G-Quadruplex Probes
Herman O. Sintim, Shizuka Nakayama

19. Lateral Flow Biosensors for the Detection of Nucleic Acid

20. Electrochemiluminescence Detection of c-Myc mRNA in Breast Cancer Cells on a Wireless Bipolar Electrode
Mei-Sheng Wu, Guang-sheng Qian, Jing-Juan Xu, Hong-Yuan Chen

21. High-Throughput Label-Free Detection of DNA Hybridization and Mismatch Discrimination Using Interferometric Reflectance Imaging Sensor
Sunmin Ahn, David S. Freedman, Xirui Zhang, M. Selim Ünlü

22. Graphene–PAMAM Dendrimer–Gold Nanoparticle Composite for Electrochemical DNA Hybridization Detection
Kumarasamy Jayakumar, Rajendiran Rajesh, Venkataraman Dharuman, Rangarajan Venkatesan

23. Advances in FISH – Front Matter

24. Application of PNA Openers for Fluorescence-Based Detection of Bacterial DNA
Irina Smolina

25. DNA Probes for FISH Analysis of C-Negative Regions in Human Chromosomes
Evgeniy S. Morozkin, Tatyana V. Karamysheva, Pavel P. Laktionov, Valentin V. Vlassov, Nikolay B. Rubtsov

26. Detection of MicroRNA – Front Matter

27. Quantitative Analysis of MicroRNA in Blood Serum with Protein-Facilitated Affinity Capillary Electrophoresis
Maxim V. Berezovski, Nasrin Khan

28. High-Throughput Functional MicroRNA Profiling Using Recombinant AAV-Based MicroRNA Sensor Arrays
Wenhong Tian, Xiaoyan Dong, Xiaobing Wu, Zhijian Wu

29. The Use of Molecular Beacons to Detect and Quantify MicroRNA
Meredith B. Baker, Gang Bao, Charles D. Searles

30. RNA Imaging in Live Cells – Front Matter

31. Sequence-Specific Imaging of Influenza A mRNA in Living Infected Cells Using Fluorescent FIT–PNA
Susann Kummer, Andrea Knoll, Andreas Herrmann, Oliver Seitz

32. Application of Caged Fluorescent Nucleotides to Live-Cell RNA Imaging
Akimitsu Okamoto

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Tags: Dmitry Kolpashchikov, Yulia Gerasimova, Nucleic Acid, Methods and Protocols