Neural Stem Cells Methods and Protocols 1st Edition by Tanja Zigova, Paul Sanberg, Juan Sanchez Ramos ISBN 9780896039643 0896039641

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Product details: 

ISBN 10:   0896039641

ISBN 13: 9780896039643

Author: Tanja Zigova, Paul R. Sanberg, Juan R. Sanchez-Ramos

In Neural Stem Cells: Methods and Protocols, internationally recognized experts from academic, clinical, and pharmaceutical laboratories describe in great detail the most frequently used cellular, molecular, and electrophysiological methods to isolate, characterize, and utilize neural stem cells. These readily reproducible techniques introduce the various sources of stem/progenitor cells, provide a wide range of conditions for their culture, and make it possible to define their properties in culture. Additional techniques are designed to help researchers identify endogenous stem cells as well as exogenous stem cells after transplantation in the brain. The protocols range from the simplest methods of isolation and characterization of neural cell properties to such very sophisticated methods as characterizing gene expression, telomerase assays, and cell cycle kinetics.

Table of contents: 

Isolation and Culture of Neural Stem Cells (NSCs)

• Neural Differentiation of Embryonic Stem Cells – K. Sue O’Shea

• Production and Analysis of Neurospheres from Acutely Dissociated and Postmortem CNS Specimens – Eric D. Laywell, Valery G. Kukekov, Oleg Suslov, Tong Zheng, Dennis A. Steindler

• Isolation of Stem and Precursor Cells from Fetal Tissue – Yuan Y. Wu, Tahmina Mujtaba, Mahendra S. Rao

• Olfactory Ensheathing Cells – Susan C. Barnett, A. Jane Roskams

• Culturing Olfactory Ensheathing Glia from the Mouse Olfactory Epithelium – Edmund Au, A. Jane Roskams

• Production of Immortalized Human Neural Crest Stem Cells – Seung U. Kim, Eiji Nakagawa, Kozo Hatori, Atsushi Nagai, Myung A. Lee, Jung H. Bang

• Adult Rodent Spinal Cord Derived Neural Stem Cells – Lamya S. Shihabuddin

• Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood – Shijie Song, J. Sanchez-Ramos

• Seeding Neural Stem Cells on Scaffolds of PGA, PLA, and Their Copolymers – Erin Lavik, Yang D. Teng, Evan Snyder, Robert Langer

Characterization of NSCs In Vitro

Cellular Techniques

• Analysis of Cell Generation in the Telencephalic Neuroepithelium – Takao Takahashi, Verne S. Caviness Jr., Pradeep G. Bhide

• Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures – Angelo L. Vescovi, Rossella Galli, Angela Gritti

• Assessing the Involvement of Telomerase in Stem Cell Biology – Mark P. Mattson, Peisu Zhang, Weiming Fu

• Detection of Telomerase Activity in Neural Cells – Karen R. Prowse

• In Vitro Assays for Neural Stem Cell Differentiation – Marcel M. Daadi

• Electron Microscopy and Lac-Z Labeling – Bela Kosaras, Evan Snyder

Electrophysiological Techniques

• Techniques for Studying the Electrophysiology of Neurons Derived from Neural Stem/Progenitor Cells – David S. K. Magnuson, Dante J. Morassutti

Molecular Techniques

• Fluorescence In Situ Hybridization

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Tags: Tanja Zigova, Paul Sanberg, Juan Sanchez Ramos, Neural Stem, Methods and Protocols