Sale!

Molecular Pathology Protocols 1st Edition by Anthony Killeen ISBN 978-0896036819 0896036812

Original price was: $50.00.Current price is: $35.00.

Molecular Pathology Protocols 1st Edition Anthony A. Killeen Digital Instant Download

Author(s): Anthony A. Killeen
ISBN(s): 9781592590810, 1592590810
Edition: 1st
File Details: PDF, 3.85 MB
Year: 2001
Language: english
SKU: EB-924756 Category: Tag:

Molecular Pathology Protocols 1st Edition by Anthony A. Killeen – Ebook PDF Instant Download/Delivery:  978-0896036819, 0896036812
Full download Molecular Pathology Protocols 1st Edition after payment

Product details: 

ISBN 10:  0896036812

ISBN 13:  978-0896036819

Author: Anthony A. Killeen

The era of molecular pathology has arrived. From its promising beg- nings in research laboratories, the field has grown, and continues to grow, to become a vital part of the care of an ever-increasing number of patients. Because of its recent emergence from the research taboratory, many molecuIar pathology protocols we still to be found in the primary litcramre, and have not appeared in a text. MO~PCU~Q~ Padhoiogy Protocob contains la- ratory protocoIs that have been developed by many of the authors for use in clinical molecular pathology laboratories and describe in detail Row to perform these assays. This book is therefore intended for clinical laboratory use by medical technologists and pathologists. It will dso be of interest to research workers who are performing these assays. In its broadest meaning, pathology is the study of disease, and therefore it follows that any disease for which the molecular basis is understood would be suitable as a topic for inclusion in this work. When seiecting protocols, it was necessary to place limits on the number of chapters that could be feasibly presented in a single work. Those protoculs that were selected are performed more frequently, or have achieved recognition as having important diagnostic utility in contemporary practice. A decision was made to exclude inherited genetic diseases with certain exceptions, such as those diseases that are associated with thrombotic states and are part of the traditional dumain of pathology.
 

Table of contents: 

I. DNA & RNA Extraction

  • DNA Extraction from Paraffin-Embedded Tissues

  • DNA Extraction from Fresh or Frozen Tissues

  • RNA Extraction from Fresh or Frozen Tissues

II. Mutation and Genotyping Analysis

  • Single-Strand Conformation Polymorphism Analysis (SSCP) of TP53 Exons 4–8

  • Cleavase® Fragment Length Polymorphism for Genotyping and Mutation Detection

  • Detection of Microsatellite Instability

  • PCR Clonality Assays Based on X-Linked Genes

III. Telomerase and Chromosomal Abnormalities

  • Telomerase Detection by In Situ Hybridization and PCR-Based Activity Assay

  • Fluorescence In Situ Hybridization (FISH)

  • HER-2/neu Amplification by FISH

IV. Cancer-Specific Molecular Diagnostics

  • Nested RT-PCR for BCR/abl

  • Detection of AML-Related Anomalies:

    • t(15;17), inv(16)/t(16;16), t(8;21)

  • Detection of BCL-2/JH Fusion in Non-Hodgkin Lymphoma

  • Breast Cancer Cell Detection Using Immunomagnetic Beads and RT-PCR

  • Molecular Detection of Circulating Prostate Cancer Cells

V. Clonality & Transplantation Monitoring

  • Detection of Clonal Gene Rearrangements in Lymphomas and Leukemias

  • Monitoring of Bone Marrow Transplant Engraftment

VI. Inherited Disease & Prenatal Testing

  • Molecular Diagnosis of:

    • Multiple Endocrine Neoplasia Type 1

    • Multiple Endocrine Neoplasia Type 2

  • Assay for I1307K Mutation in APC Gene

  • Prenatal RhD Genotyping for Hemolytic Disease of the Newborn

  • Molecular Diagnosis of Hereditary Thrombotic Disorders

  • Molecular Diagnosis of Hereditary Hemochromatosis

VII. Infectious Disease Detection

  • Human Papillomavirus Detection via PCR and In Situ Hybridization

  • Epstein-Barr Virus Detection (Parts I, II, III)

  • Kaposi’s Sarcoma-Associated Herpesvirus / HHV-8 Detection

  • Cytomegalovirus: Quantitative PCR Applications

  • Colorimetric PCR for HSV Detection and Typing

  • Hepatitis C Virus Detection and Typing

  • Mycobacteria Detection in Paraffin-Embedded Tissues

  • HIV-1 RNA Quantitation via AMPLICOR and COBAS Tests

VIII. Pharmacogenetics and Risk Marker Genotyping

  • Apolipoprotein E Genotyping

  • Genotyping CYP2D6*4 Mutation Using TaqMan Probes

People also search for:

molecular pathology procedures
    
molecular protocols
    
molecular pathology examples
    
molecular pathology course
    
a molecular pathology

Tags: Anthony Killeen, Molecular Pathology