Microfluidics Diagnostics Methods and Protocols 1st Edition by Valérie Taly, Stéphanie Descroix, Karla Perez Toralla ISBN 978-1071638507 1071638507

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Product details: 

ISBN 10:1071638507 

ISBN 13:  978-1071638507

Author: Valérie Taly, Stéphanie Descroix, Karla Perez-Toralla

This detailed volume explores a wide range of microfluidic-based approaches that exploit the unique features of microfluidic devices, which hold significant potential in the field of diagnosis. Beginning with a section on microchips for sample preparation, including cell-free DNA, exosomes, and cells, the book continues by covering protein marker analysis and detection, single-cell analysis, analysis of bacteria and viruses, as well as human cell-based culture and analysis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Microfluidics Diagnostics: Methods and Protocols serves as an ideal guide for researchers investigating how microfluidics can shape the future of diagnosis.

Table of contents: 

PART I

OVERVIEW

1 Self-Powered Microfluidics for Point-of-Care Solutions: From Sampling to Detection of Proteins and Nucleic Acids

Dries Vloemans, Lorenz Van Hileghem, Henry Ordutowski, Francesco Dal Dosso, Dragana Spasic, and Jeroen Lammertyn

PART II

MICROCHIPS FOR SAMPLE PREPARATION INCLUDING CELL-FREE DNA, EXOSOMES, AND CELLS

2 DNA Sizing with pg/µL. Sensitivity for Cell-Free DNA Analysis with the µLAS Technology..

Audrey Boutonnet, Inga Tijunelyte, Aurélien Bancaud, and Frédéric Ginot

3 On-Chip Magnetic Extraction of Circulating Cell-Free DNA from Biological Samples

Karla Perez-Toralla, Iago Pereiro, Sonia Garrigou, Fahima Di Federico, Monica Araya-Farias, Charlotte Proudhon, François-Clément Bidard, Jean-Louis Viovy, Valérie Taly, and Stéphanie Descroix

4 Isolation of Extracellular Vesicles from Cell Culture and Blood Through Nano-Targeted DLD Microfluidic Device..

Marie Gaillard, Nicolas Sarrut-Rio, Catherine Pudda, François Boizot, Yoann Roupioz, and Aurélie Thuaire

5 Microchip for Immunomagnetic Sorting of Circulating Tumor Cells (CTCs)

Lucie Descamps, Emmanuelle Laurenceau, Sophie Cavassila, Léa Payen, Damien Le Roy, and Anne-Laure Deman

PART III MICROCHIPS FOR PROTEIN MARKERS ANALYSIS/DETECTION

6 Enhancing the Efficiency of Conventional Surface Immunoassays Within Standard Labware Using Microscale Flows

Iago Pereiro, Anna Fomitcheva-Khartchenko, and Govind V. Kaigala

7 Droplet Microfluidics with Capillary Electrophoresis for Glycan Biomarker Analysis.

Théo Liénard-Mayor, Camille Bricteux, Nguyet-Thuy Tran, Arnaud Bruneel, Myriam Taverna, and Thanh Duc Mai

8 Microfluidic Cartridge for Bead-Based Affinity Assays

Inés F. Pinto, Veronique Chotteau, and Aman Russom

PART IV MICROCHIPS FOR SINGLE CELL ANALYSIS

9 A Guide to the Quantitation of Protein Secretion Dynamics at the Single-Cell Level.

Nathan Aymerich, Olivia T. M. Bucheli, Kevin Portmann, Klaus Eyer, and Jean Baudry

10 Magnetic Microtweezers for High-Throughput Bioseparation in Sub-Nanoliter Droplets

Simon Dumas, Lucile Alexandre, Mathilde Richerd, Marco Serra, and Stéphanie Descroix

PART V MICROFLUIDIC CHIPS FOR ANALYSIS OF BACTERIA AND VIRUS

11 A Thermoplastic Microsystem to Perform Antibiotic Susceptibility Testing by Monitoring Oxygen Consumption..

Petra Jusková, André Kling, Steven Schmitt, and Petra S. Dittrich

12 An Immiscible-Phase Filtration Device for Isolation, Amplification, and Detection of Nucleic Acids for Clinical Diagnostics

Bongkot Ngamsom, Pablo Rodriguez-Mateos, and Nicole Pamme

PART VI

MICROFLUIDIC CHIPS FOR HUMAN CELL-BASED CULTURE AND ANALYSIS

13 Microfluidic 3D Cytotoxic Assay.

Hyeri Choi, Sunghun Cheong, Ailian Jin, Dohyun Park, and Noo Li Jeon

14 Evaluating Nanoparticles Penetration by a New Microfluidic Hydrogel-Based Approach

Saba Goodarzi, François Lux, and Charlotte Rivière

15 Selective Targeting of Tumor Cells in a Microfluidic Tumor Model with Multiple Cell Types.

Bas van de Crommert, Valentina Palacio-Castañeda, and Wouter P. R. Verdurmen

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Tags: Valérie Taly, Stéphanie Descroix, Karla Perez Toralla, Microfluidics Diagnostics, Methods and Protocols