MHC Protocols 1st Edition by James Stephen Powis, Robert Vaughan ISBN 978-1592592913 1592592913

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Product details: 

ISBN 10:  1592592913

ISBN 13: 978-1592592913

Author:  James Stephen H. Powis, Robert W. Vaughan

The aim of MHC Protocols is to document protocols that can be used for the analysis of genetic variation within the human major histocompatibility complex (MHC; HLA region). The human MHC encompasses approximately 4 million base pairs on the short arm of chromosome 6 at cytogenetic location 6p21. 3. The region is divided into three subregions. The telomeric class I region contains the genes that encode the HLA class I molecules HLA-A, -B, and -C. The centromeric class II region contains the genes encoding the HLA class II molecules HLA-DR, -DQ, and -DP. In between is the class III region, originally identified because it contains genes encoding components of the complement pathway. The entire human MHC has recently been sequenced (1) and each subregion is now known to contain many other genes, a number of which have immunological functions. The study of polymorphism within the MHC is well established, because the region contains the highly polymorphic HLA genes. HLA polymorphism has been used extensively in solid organ and bone marrow transplantation to match donors and recipients. As a result, large numbers of HLA alleles have been identified, a process that has been further driven by recent interest in HLA gene diversity in ethnic populations. The extreme genetic variation in HLA genes is believed to have been driven by the evolutionary response to infectious agents, but relatively few studies have analyzed associations between HLA genetic variation and infectious disease, which has been difficult to demonstrate.

Table of contents: 

1 Front Matter
2 HLA Informatics
3 Accessing HLA Sequencing Data Through the 6ace Database
4 Polymorphism in Classical and Nonclassical Hla Genes
5 Front Matter
6 HLA Typing by Restriction Fragment Length Polymorphism Analysis
7 PCR-Restriction Fragment Length Polymorphism Typing of Class I and II Alleles
8 PCR-Sequence-Specific Oligonucleotide Probe Typing for HLA-A, -B, and -DR
9 HLA-DPA1 and -DPB1 Typing Using the PCR and Nonradioactive Sequence-Specific Oligonucleotide Probes
10 PCR-Sequence-Specific Primer Typing of HLA Class I and Class II Alleles
11 HLA Typing With Reference Strand-Mediated Conformation Analysis
12 Sequencing Protocols for Detection of HLA Class I Polymorphism
13 HLA-E and HLA-G Typing
14 Typing Alleles of HLA-DM
15 Polymorphism in Non-Hla Mhc Genes
16 Front Matter
17 Typing Alleles of TAP1 and TAP2
18 Determining Alleles of the C2 Gene by Southern Blotting
19 Complement C4 Protein and DNA Typing Methods
20 Typing of Tumor Necrosis Factor Alleles
21 Molecular Typing of the MHC Class I Chain-Related Gene Locus
22 Microsatellites
23 Front Matter
24 HLA Microsatellite Analysis
25 Back Matter

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Tags: James Stephen Powis, Robert Vaughan, MHC Protocols