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(Ebook PDF) Metagenomics Methods and Protocols 1st Edition by Wolfgang Streit, Rolf Daniel ISBN 9781607618232 1607618230 full chapters

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Metagenomics Methods and Protocols 1st Edition José M. Vieites Digital Instant Download

Author(s): José M. Vieites, María-Eugenia Guazzaroni, Ana Beloqui, Peter N. Golyshin (auth.), Wolfgang R. Streit, Rolf Daniel (eds.)
ISBN(s): 9781607618232, 1607618230
Edition: 1
File Details: PDF, 5.18 MB
Year: 2010
Language: english
SKU: EB-4288606 Category: Tags: , , , , ,

(Ebook PDF) Metagenomics Methods and Protocols 1st Edition by Wolfgang Streit, Rolf Daniel  -Ebook PDF Instant Download/Delivery:9781607618232, 1607618230 

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ISBN 10:1607618230 

ISBN 13:9781607618232

Author: Wolfgang R. Streit, Rolf Daniel 

The world of microorganisms encompasses a vast diversity of living organisms, each possessing unique genes, cellular components, and metabolic reactions that interact within the cell and communicate with the environment in diverse ways. Understanding and predicting ecosystem functioning requires a broader perspective on these interconnected cellular and environmental processes, achievable through a systems microbiology approach.  

Simultaneously, the rise of metagenomics offers an innovative tool for studying communities of uncultured microorganisms. This review surveys key methodologies in metagenomics and explores systems microbiology approaches for gaining a mechanistic understanding of complex microbial systems. It examines compositional, evolutionary, and metabolic properties, highlighting how metagenomics serves as a holistic indicator of ecosystem responses, encompassing matter, nutrient, energy sources, and functional networking.

Table of Contents:

  1. Construction of Small-Insert and Large-Insert Metagenomic Libraries

  2. Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a Universal cDNA as Universal Template for Marker Gene Studies

  3. Functional Metagenomics as a Tool to Tap into Natural Diversity of Valuable Biotechnological Compounds

  4. Functional and Sequence-Specific Screening Protocols for the Detection of Novel Antimicrobial Resistance Genes in Metagenomic DNA

  5. Identification of PKS Gene Clusters from Metagenomic Libraries Using a Next-Generation Sequencing Approach

  6. Activity-Based Screening of Metagenomic Fosmid Libraries for Hydrogen-Uptake Enzymes

  7. Functional Metagenomics Approach for the Discovery of Novel Genes Encoding Phosphatase Activity

  8. Isolation of Genes Encoding Carbon Metabolism Pathways from Complex Microbial Communities

  9. Screening Metagenomics for Algae Cell Wall Carbohydrates Degrading Hydrolases in Enrichment Cultures

  10. The PET-Degrading Potential of Global Metagenomes: From In Silico Mining to Active Enzymes

  11. High-Throughput Screening for Thermostable Polyester Hydrolases

  12. Metagenomic Screening of a Novel PET Esterase via In Vitro Expression System

  13. Assigning Functions of Unknown Enzymes by High-Throughput Enzyme Characterization

  14. Isolation of a Host-Confined Phage Metagenome Allows the Detection of Phages Both Capable and Incapable of Plaque Formation

  15. CRISPR-Cas9 Shaped Viral Metagenomes Associated with Bacillus subtilis

  16. Cloning and Expression of Metagenomic DNA in Streptomyces lividans and Its Subsequent Fermentation for Optimized Production

  17. DNA-, RNA-, and Protein-Based Stable-Isotope Probing for High-Throughput Biomarker Analysis of Active Microorganisms

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Wolfgang Streit,Rolf Daniel,Metagenomics,Methods,Protocols