Metabolic Analysis Using Stable Isotopes First Edition by Christian Metallo ISBN 0128025345 978-0128025345
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Product details:
ISBN 10: 0128025345
ISBN 13: 978-0128025345
Author: Christian Metallo
Metabolic Analysis Using Stable Isotopes, the newest volume in Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in metabolic analysis using stable isotopes.
– Continues the legacy of this premier serial with quality chapters on metabolic analysis using stable isotopes
– Represents the newest volume in Methods in Enzymology, providing a premier serial with quality chapters authored by leaders in the field
– Ideal reference for those interested in the study of metabolism, metabolic tracing, isotopic labeling, and lipogenesis
Table of contents:
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Introduction
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¹³C MRS of DNP Probes: Current Approaches to Studying Metabolism
2.1 DNP Probes for In Vivo Applications: An Overview
2.2 The “Star Probe”: [1-¹³C] Pyruvate
2.3 Probing Enzymatic Pathways in Living Systems
2.4 Applications to Other Physiological Processes -
Requirements of ¹³C DNP Probes
3.1 Biological Considerations
3.2 Chemical Considerations
3.3 Characterization of the ¹³C DNP Probe -
Conducting Hyperpolarized Experiments
4.1 Main Components of a DNP-MR Laboratory
4.2 In Vitro Experiments
4.3 Live Cell Experiments
4.4 Ex Vivo Experiments
4.5 Preclinical In Vivo Experiments -
Acquisition Strategies for Hyperpolarized MR Spectroscopy and Imaging
5.1 In Vitro Hyperpolarized ¹³C MR Studies
5.2 Hyperpolarized ¹³C MR Studies on Cells or Perfused Organs
5.3 In Vivo Hyperpolarized ¹³C MR Studies -
Analysis of Hyperpolarized MR Data
6.1 Relative Quantification
6.2 Quantification Using Modeling -
Summary and Conclusion
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Tags: Christian Metallo, Metabolic Analysis, Using Stable