Membrane Protein Purification and Crystallization A Practical Guide 2nd Edition by Carola Hunte, Gebhard von Jagow, Hermann Schagger ISBN 0123617766 978-0123617767

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Product details: 

ISBN 10: 0123617766 

ISBN 13: 978-0123617767

Author: Carola Hunte, Gebhard von Jagow, Hermann Schagger

This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins.

Table of contents: 

I. Strategies and Techniques
Purification Strategies for Membrane Proteins
Chromatographic Techniques and Basic Operations in Membrane Protein Purification
Production and purification of recombinant membrane proteins
SDS Electrophoresis Techniques
Blue-Native Electrophoresis
Preparative Isoelectric Focussing
Membrane protein crystallization

II. Discussion of Selected Isolation Protocols
Lipid Dependent Inactivation and Reactivation of Bovine complex III
Purification of affinity-epitope tagged G-protein coupled receptors
Purification of NhaA, the Na+/H+ Antiporter of Escherichia coli for 3D or 2D
Crystallization
Purification of the yeast cytochrome bc1 complex

III. Crystallization of Membrane Proteins
Antibody fragment-mediated crystallization of membrane proteins
Crystallization of Wolinella succinogenes quinol:fumarate reductase
Ba3-type cytochrome-c oxidase from Thermus thermophilus
purification, crystallization, and crystal transformation
Two-dimensional crystallization of membrane proteins: a practical guide
In cubo crystallization of membrane proteins

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