Lymphoma Methods and Protocols 1st Edition by Claire Dallman, Graham Packham ISBN 9781592599363 1592599362

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Product details: 

ISBN 10: 1592599362

ISBN 13: 9781592599363

Author: Claire Dallman, Graham Packham

The book Lymphoma: Methods and Protocols is part of the Methods in Molecular Medicine series (Volume 115) and was published by Humana Press in 2005. It is edited by Tim Illidge and Peter W.M. Johnson. Claire Dallman contributed a chapter titled “Purification of Primary Malignant B-Cells and Immunoblot Analysis of Bcl-2 Family Proteins,” co-authored with Graham Packham. 

Utilizing immunoglobulin gene rearrangements as clonality markers

Analyzing somatic mutations in variable regions to determine transformation stages

Applying gene arrays to assess biological heterogeneity in morphologically similar diseases

Employing DNA microarrays and immunoglobulin V-gene rearrangements

Measuring minimal residual disease

Developing DNA vaccines with patient-specific sequences derived from the lymphoma
Each protocol follows the established Methods in Molecular Medicine format, providing step-by-step laboratory instructions, introductions outlining the principles behind the techniques, lists of necessary equipment and reagents, and troubleshooting tips.

 

Table of contents: 

1 Noncontact Laser Microdissection and Pressure Catapulting: Sample Preparation for Genomic, Transcriptomic, and Proteomic Analysis

Yilmaz Niyaz, Monika Stich, Bernd Sägmüller, Renate Burgemeister, Gabriele Friedemann, Ulrich Sauer, Rainer Gangnus, and Karin Schütze

2 Applications of the Universal DNA Microarray in Molecular Medicine Reyna Favis, Norman P. Gerry, Yu-Wei Cheng, and Francis Barany…

3 Sensitive Detection of SARS Coronavirus RNA by a Novel Asymmetric Multiplex Nested RT-PCR Amplification Coupled With Oligonucleotide Microarray Hybridization Zhi-wei Zhang, Yi-ming Zhou, Yan Zhang, Yong Guo, Sheng-ce Tao, Ze Li, Qiong Zhang, and Jing Cheng

4 Genotyping Single-Nucleotide Polymorphisms by Minisequencing Using Tag Arrays Lovisa Lovmar and Ann-Christine Syvänen

5 Single-Nucleotide Polymorphism and Mutation Identification by the Nanogen Microelectronic Chip Technology

Maurizio Ferrari, Laura Cremonesi, Pierangelo Bonini, Barbara Foglieni, and Stefania Stenirri

6 Molecular Diagnostic Testing for Inherited Thrombophilia Using Invader® Margaret A. Keller.

7 Controlled Agitation During Hybridization: Surface Acoustic Waves Are Shaking Up Microarray Technology

Achim Wixforth

8 Rapid Screening for 31 Mutations and Polymorphisms in the Cystic Fibrosis Transmembrane Regulator Gene by Luminex® XMAP™™ Suspension Array

Sherry A. Dunbar and James W. Jacobson

9 Protein Microarray-Based Screening of Antibody Specificity

Rhonda Bangham, Gregory A. Michaud, Barry Schweitzer, and Paul F. Predki

10 Multiplexed Protein Analysis Using Antibody Microarrays and Label-Based Detection

Brian B. Hope

11 Allergen Microarrays

Tito Bacarese-Hamilton, Julian Gray, Andrea Ardizzoni, and Andrea Crisanti

12 Enhanced Protein Profiling Arrays for Quantitative Measurement of Protein Expression in Multiple Samples

Ruochun Huang, Qian Shi, Weimin Yang, and Ruo-Pan Huang

13 Multiplexed Cytokine Sandwich Immunoassays:

Clinical Applications

Uma Prabhakar, Edward Eirikis, Bruce E. Miller, and Hugh M. Davis

14 Validation and Quality Control of Protein Microarray-Based Analytical Methods

Larry J. Kricka and Stephen R. Master.

15 Tissue Microarrays

Ronald Simon, Martina Mirlacher, and Guido Sauter

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