Host Pathogen Interactions Methods and Protocols 1st Edition by Christof Hauck, Steffen Rupp, Kai Sohn ISBN 978-1588298867 1588298867

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ISBN 10: 1588298867

ISBN 13:  978-1588298867

Author:  Christof R. Hauck, Steffen Rupp, Kai Sohn  

This volume presents a comprehensive collection of laboratory protocols focused on deciphering the molecular-level interactions between hosts and pathogens—from bacteria and fungi to protozoan parasites. It emphasizes experimental methods designed to probe mechanisms of infection, virulence, immune evasion, and host responses.

Table of contents: 

PART I: BACTERIAL PATHOGENS

1 Introduction: Bacterial Pathogens

Christof R. Hauck

2 Transcriptome Analyses in the Interaction of Neisseria meningitidis with Mammalian Host Cells

Alexandra Schubert-Unkmeir, Anja Schramm-Glück, Matthias Frosch, and Christoph Schoen

3 Surface-Exposed Adherence Molecules of Streptococcus pneumoniae Sven Hammerschmidt

4 Screening of Virulence Traits in Legionella pneumophila and Analysis of the Host Susceptibility to Infection by Using the Dictyostelium Host Model System Olaga Shevchuk and Michael Steinert

5 Characterizing Host Receptor Recognition by Individual Bacterial Pathogens Katharina Kuespert and Christof R. Hauck

PART II: FUNGAL PATHOGENS

6 Introduction: Fungal Pathogens

Steffen Rupp

7 Isolation and Purification of Antigenic Components of Cryptococcus

Karen L. Wozniak and Stuart M. Levitz

8 A Method for Examining Glycans Surface Expression of Yeasts by Flow Cytometry

Maria Martínez-Esparza, Aurore Sarazin, Daniel Poulain, and Thierry Jouault…

9 Human Epithelial Model Systems for the Study of Candida Infections

In Vitro: Part I. Adhesion to Epithelial Models

Kai Sohn and Steffen Rupp

10 Human Epithelial Model Systems for the Study of Candida Infections In Vitro: Part II. Histologic Methods for Studying Fungal Invasion

Rosa Hernandez and Steffen Rupp

11 In Vitro Systems for Studying the Interaction of Fungal Pathogens with Primary Cells from the Mammalian Innate Immune System

Christelle Bourgeois, Olivia Majer, Ingrid Frohner, and Karl Kuchler

12 Experimental Infection of Rodent Mammals for Fungal Virulence Testing Anja Schweizer and Klaus Schröppel

13 Real-Time and Semiquantitative RT-PCR Methods to Analyze Gene Expression Patterns During Aspergillus-Host Interactions Jin Woo Bok, Nancy P. Keller, and Dimitrios I. Tsitsigiannis

14 Antibody-Based Strategy to Identify Candida albicans Genes Expressed During Infections Cornelius J. Clancy, Shaoji Cheng, and M. Hong Nguyen

15 Identification of the Candida albicans Immunome During Systemic Infection by Mass Spectrometry Aida Pitarch, César Nombela, and Concha Gil

PART III: PARASITES

16 Introduction: Parasites Klaus Brehm and Carsten G. K. Lüder

17 Axenic In Vitro Cultivation of Echinococcus multilocularis Metacestode Vesicles and the Generation of Primary Cell Cultures Markus Spiliotis and Klaus Brehm

18 Culture of Exoerythrocytic Stages of the Malaria Parasites Plasmodium falciparum and Plasmodium vivax Liwang Cui, Namtip Trongnipatt, Jetsumon Sattabongkot, and Rachance Udomsangpetch

19 Modulation of Caspase Activation by Toxoplasma gondii Diana Hippe, Andrea Gais, Uwe Gross, and Carsten GK Lüder

PART IV: HOST RESPONSES

20 Introduction: Host Responses

Martin Schaller, Günther Weindl, and Bernhard Hube

21 Fungal and Bacterial Killing by Neutrophils David Ermert, Arturo Zychlinsky, and Constantin Urban

22 Endothelial Cell Stimulation by Candida albicans Quynh T. Phan and Scott G. Filler

23 Models of Oral and Vaginal Candidiasis Based on In Vitro Reconstituted Human Epithelia for the Study of Host-Pathogen Interactions Martin Schaller and Günther Weindl…….

24 Phagocytosis of Candida albicans by RNAi-Treated Drosophila S2 Cells Shannon L. Stroschein-Stevenson, Edan Foley, Patrick H. O’Farrell, and Alexander D. Johnson

25 Oral Mucosal Cell Response to Candida albicans in Transgenic Mice Expressing HIV-1 Louis de Repentigny, Daniel Lewandowski, Francine Aumont, Zaher Hanna, and Paul Jolicoeur

26 Proteomic Profiling of Serologic Response to Candida albicans During Host-Commensal and Host-Pathogen Interactions Aida Pitarch, César Nombela, and Concha Gil

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Tags: Christof Hauck, Steffen Rupp, Kai Sohn, Host Pathogen Interactions, Methods and Protocols