Hepatocytes Methods and Protocols 1st Edition by Patrick Maurel ISBN 978-1607616887 1607616887

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Product details: 

ISBN 10:  1607616887

ISBN 13:  978-1607616887

Author: Patrick Maurel

Hepatocytes account for approximately 80% of the liver mass and play a significant role in various aspects of liver physiopathology, exhibiting unrivaled complexity and diversity of functions. In Hepatocytes: Methods and Protocols, expert researchers provide the reader with methods, technical protocols, and review chapters focusing on selected areas of hepatocyte biology including isolation, culture, differentiation and stem cells, and hepatocyte use in clinical, basic, and applied research. With a specific emphasis on human hepatocytes, the volume presents chapters covering subjects including hepatocyte culture models, cryopreservation methods, differentiation assessment, liver ontogenesis, production of hepatocytes from stem cells, drug/xenobiotic metabolism, toxicity and transport, bile acid and blood coagulation factor production, infection by HBV and HCV, humanized animals, biortificial liver devices, hepatocyte transplantation. As a volume in the highly successful Methods in Molecular Biology™ series, protocol chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Hepatocytes: Methods and Protocols will be useful to all those who are currently using or planning to use human, or animal, hepatocytes to investigate any aspect of liver physiopathology or who are interested in liver development or liver stem cells and liver biotherapy.

Table of contents: 

1. General Review on In Vitro Hepatocyte Models and Their Applications
   Christiane Guguen-Guillouzo, Andre Guillouzo

2. Human Foetal Hepatocytes: Isolation, Characterization, and Transplantation
   Anne Weber, Thomas Touboul, Sylvie Mainot, Julie Branger, Dominique Mahieu-Caputo

3. Isolation and Culture of Primary Hepatocytes from Resected Human Liver Tissue
   Edward L. LeCluyse, Eliane Alexandre

4. Optimisation of the Cryopreservation of Primary Hepatocytes
   Nicola J. Hewitt

5. Cryopreservation of Human Hepatocytes for Clinical Use
   Robin D. Hughes, Ragai R. Mitry, Sharon C. Lehec

6. Hepatocyte Differentiation
   Katy M. Olsavsky Goyak, Elizabeth M. Laurenzana, Curtis J. Omiecinski

7. Reversible Manipulation of Apoptosis Sensitivity in Cultured Hepatocytes by Matrix-Mediated Manipulation of Signaling Activities
   Patricio Godoy, Markus Schug, Alexander Bauer, Jan G. Hengstler

8. Markers and Signaling Factors for Stem Cell Differentiation to Hepatocytes: Lessons from Developmental Studies
   Frédéric Lemaigre

9. Hepatic Stem Cells
   Robert E. Schwartz, Catherine Verfaillie

10. Hepatic Stem Cells and Liver Development
    Nalu Navarro-Alvarez, Alejandro Soto-Gutierrez, Naoya Kobayashi

11. Generation of Hepatocytes from Human Embryonic Stem Cells
    Neta Lavon

12. Isolation and Culture of Adult Human Liver Progenitor Cells: In Vitro Differentiation to Hepatocyte-Like Cells
    Sabine Gerbal-Chaloin, Cédric Duret, Edith Raulet, Francis Navarro, Pierre Blanc, Jeanne Ramos et al.

13. The HepaRG Cell Line: Biological Properties and Relevance as a Tool for Cell Biology, Drug Metabolism, and Virology Studies
    Marie-Jeanne Marion, Olivier Hantz, David Durantel

14. Transdifferentiation of Pancreatic Cells to Hepatocytes
    Chia-Ning Shen, David Tosh

15. Evaluation of Drug Metabolism, Drug–Drug Interactions, and In Vitro Hepatotoxicity with Cryopreserved Human Hepatocytes
    Albert P. Li

16. The Use of Human Hepatocytes to Investigate Drug Metabolism and CYP Enzyme Induction
    Sylvie Klieber, François Torreilles, François Guillou, Gérard Fabre

17. The Use of Hepatocytes to Investigate UDP-Glucuronosyltransferases and Sulfotransferases
    Sylvie Fournel-Gigleux, Michael W.H. Coughtrie, Mohamed Ouzzine, Jacques Magdalou

18. The Use of Hepatocytes to Investigate Drug Uptake Transporters
    Kazuya Maeda, Yuichi Sugiyama

19. Metabonomic Studies on Human Hepatocyte in Primary Culture

20. The Application of HepRG Cells in Evaluation of Cytochrome P450 Induction Properties of Drug Compounds
    Tommy B. Andersson

21. The Use of Hepatocytes to Investigate Drug Toxicity
    María José Gómez-Lechón, José V. Castell, María Teresa Donato

22. The Use of Human Hepatocytes to Investigate Bile Acid Synthesis
    Ewa C. S. Ellis, Lisa-Mari Nilsson

23. Use of Human Hepatocytes to Investigate Blood Coagulation Factor
    Christine Biron-Andréani, Edith Raulet, Lydiane Pichard-Garcia, Patrick Maurel

24. Use of Human Hepatocytes to Investigate HCV Infection
    Lydiane Pichard-Garcia, Philippe Briolotti, Dominique Larrey, Antonio Sa-Cunha, Bertrand Suc, Sylvain Laporte et al.

25. The Use of Hepatocytes to Investigate HDV Infection: The HDV/HepaRG Model
    Camille Sureau

26. Rodent Models of Liver Repopulation
    Helène Gilgenkrantz

27. Chimeric Mice with Humanized Liver: Tools for the Study of Drug Metabolism, Excretion, and Toxicity
    Stephen C. Strom, Julio Davila, Markus Grompe

28. Bioartificial Liver Support Systems
    Gesine Pless

29. Human Hepatocyte Transplantation

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