Guide to Research Techniques in Neuroscience Second Edition by Matt Carter, Jennifer Shieh ISBN 0128005114 978-0128005118
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ISBN 10: 0128005114
ISBN 13: 978-0128005118
Author: Matt Carter, Jennifer C. Shieh
Neuroscience is, by definition, a multidisciplinary field: some scientists study genes and proteins at the molecular level while others study neural circuitry using electrophysiology and high-resolution optics. A single topic can be studied using techniques from genetics, imaging, biochemistry, or electrophysiology. Therefore, it can be daunting for young scientists or anyone new to neuroscience to learn how to read the primary literature and develop their own experiments.
This volume addresses that gap, gathering multidisciplinary knowledge and providing tools for understanding the neuroscience techniques that are essential to the field, and allowing the reader to design experiments in a variety of neuroscience disciplines.
- Written to provide a “hands-on” approach for graduate students, postdocs, or anyone new to the neurosciences
- Techniques within one field are compared, allowing readers to select the best techniques for their own work
- Includes key articles, books, and protocols for additional detailed study
- Data analysis boxes in each chapter help with data interpretation and offer guidelines on how best to represent results
- Walk-through boxes guide readers step-by-step through experiments
Table of contents:
Chapter 1. Whole-Brain Imaging
Structural Brain Imaging Techniques
Functional Brain Imaging Techniques
Functional Imaging Experimental Design and Analysis
Conclusion
Chapter 2. Animal Behavior
Considerations for Choosing and Performing a Behavioral Assay
Rodent Behavioral Paradigms
Drosophila Behavioral Paradigms
Caenorhabditis elegans Behavioral Paradigms
Nonhuman Primate Behavioral Paradigms
Conclusion
Chapter 3. Stereotaxic Surgeries and In Vivo Techniques
Stereotaxic Surgeries
Implants for Long-Term Access to the Brain
Manipulating Neural Activity In vivo
Conclusion
Chapter 4. Electrophysiology
A Brief Review of the Electrical Properties of Neurons
The Electrophysiology Rig
Categories of Electrophysiology Recordings
Electrophysiology Tissue Preparations
Manipulating Neural Activity during Electrophysiology Experiments
Conclusion
Chapter 5. Microscopy
Essential Principles of Microscopy
Light Microscopy
Fluorescence Microscopy
Electron Microscopy
Preparing and Interpreting Microscopy Data
Conclusion
Chapter 6. Visualizing Neural Structure
Tissue Preparation
Visualizing Morphology
Visualizing Gene and Protein Expression
Visualizing Circuitry
Conclusion
Chapter 7. Visualizing Neural Function
Static Markers of Activity
Visualizing Neural Activity
Visualizing Protein Function
Conclusion
Chapter 8. Manipulating Neural Activity
Experimental Logic of Manipulating Neural Activity
Physical Manipulation
Electrical Manipulation
Pharmacological Manipulation
Genetic Manipulation
Pharmacogenetic Manipulation
Optogenetic Manipulation
Neuromodulation Techniques Used in Humans
Conclusion
Chapter 9. Identifying Genes and Proteins of Interest
A Brief Review of Genes and Proteins
Genetic Screens
In silico Screens
Molecular Screens
Conclusion
Chapter 10. Molecular Cloning and Recombinant DNA Technology
Isolating DNA Fragments
Cloning DNA
Identifying DNA
Conclusion
Chapter 11. Gene Delivery Strategies
Physical Gene Delivery
Chemical Gene Delivery
Viral Gene Delivery
Conclusion
Chapter 12. Making and Using Transgenic Organisms
Transgenes
The Transgenic Construct
Binary Transgenic Systems
Making Transgenic Organisms
Conclusion
Chapter 13. Manipulating Endogenous Genes
Classical Gene Targeting Methods
Nuclease-Based Gene Targeting Methods
Disrupting Gene Products
Conclusion
Chapter 14. Cell Culture Techniques
Cell Culture Equipment and Reagents
Immortalized Cell Lines
Primary Cell and Tissue Culture
Stem Cell Cultures
Manipulating Cells in Culture
Conclusion
Chapter 15. Biochemical Assays and Intracellular Signaling
Introduction to Signal Transduction and Intracellular Signaling
Fundamental Tools Used to Study Proteins
Investigating Protein Expression
Investigating Protein–Protein Interactions
Investigating Posttranslational Modifications
Investigating Protein–DNA Interactions
Conclusion
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Tags: Matt Carter, Jennifer Shieh, Guide to Research, in Neuroscience