Glycomics 1st Edition by Minoru Fukuda- Ebook PDF Instant Download/Delivery: 0121828212, 978-0121828219
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ISBN 10: 0121828212
ISBN 13: 978-0121828219
Author: Minoru Fukuda
In the past decade, there has been an explosion of progress in understanding the roles of carbohydrates in biological systems. This explosive progress was made with the efforts in determining the roles of carbohydrates in immunology, neurobiology and many other disciplines, examining each unique system and employing new technology. This volume represents the second of three in the Methods in Enzymology series, including Glycobiology (vol. 415) and Functional Glycomics (vol. 417), dedicated to disseminating information on methods in determining the biological roles of carbohydrates. These books are designed to provide an introduction of new methods to a large variety of readers who would like to participate in and contribute to the advancement of glycobiology. The methods covered include structural analysis of carbohydrates, biological and chemical synthesis of carbohydrates, expression and determination of ligands for carbohydrate-binding proteins, gene expression profiling including micro array, and generation of gene knockout mice and their phenotype analyses.
Table of contents:
Section I. Carbohydrate–Protein Interactions–Physical Measurements
Characterization of Specific Donor Binding to α1,4-N-Acetylhexosaminyltransferase EXTL2 Using Isothermal Titration Calorimetry
Mack SobhanY and Masahiko Negishi
NMR Analysis of Carbohydrate–Protein Interactions
Jesus Angulo, Christoph Rademacher, Thorsten Biet, Andrew J. Benie, Astrid Blume, Hannelore Peters, Monica Palcic, Francisco Parra, and Thomas Peters
X-ray Crystal Structure Determination of Mammalian Glycosyltransferases
John E. Pak and James M. Rini
Section II. Phage Display Library
Screening of Peptide-Displaying Phage Libraries to Identify Short Peptides Mimicking Carbohydrates
Michiko N. Fukuda
Phage Display-Derived Human Antibodies Against Specific Glycosaminoglycan Epitopes
Nicole C. Smits, Joost F. M. Lensen, Tessa J. M. Wunhoven, Gerdy B. Ten Dam, Guido J. Jenniskens, and Toin H. Van Kuppevelt
Section III. Glycosyltransferase Expression Profiling
Comprehensive Enzymatic Characterization of Glycosyltransferases with a β3GT and β4GT Motif
Akira Togayachi, Takashi Sato, and Hisashi Narimatsu
Autofluorescent Proteins for Monitoring the Intracellular Distribution of Glycosyltransferases
Assou El-Battari
Expression Profiling of Glycosyltransferases and Related Enzymes Using In Situ Hybridization
Jun Nakayama, Misa Suzuki, Masami Suzuki, and Minoru Fukuda
Expression Profiling of Glycosyltransferases Using RT-PCR
Motohiro Kobayashi
Expression Profiling of Glycosyltransferases and Related Enzymes Using Gene Microarrays
Jennifer A. Hammond and Steven R. Head
Section IV. CHO Mutants in Glycan Biosynthesis
Lectin-Resistant CHO Glycosylation Mutants
Santosh Kumar Patnaik and Pamela Stanley
CHO Glycosylation Mutants: GPI Anchor
Yusuke Maeda, Hisashi Ashida, and Taroh Kinoshita
CHO Glycosylation Mutants: Proteoglycans
Lijuan Zhang, Roger Lawrence, Beth A. Frazier, and Jeffrey D. Esko
Section V. Function of Sulfation
Determination of Substrate Specificity of Sulfotransferases and Glycosyltransferases (Proteoglycans)
Hiroko Habuchi, Osami Habuchi, Kenji Uchimura, Koji Kimata, and Takashi Muramatsu
Measuring the Activities of the Sulfs: Two Novel Heparin/Heparan Sulfate Endosulfatases
Kenji Uchimura, Megumi Morimoto-Tomita, and Steven D. Rosen
16. Determination of Chemokine–Glycosaminoglycan Interaction Specificity
Hiroto Kawashima
17. Identification of Proteoglycan-Binding Proteins
Takashi Muramatsu, Hisako Muramatsu, and Tetsuhiro Kojima
18. Functions of Glycans Revealed by Gene Inactivation of L-Selectin Ligand Sulfotransferases in Mice
Hiroto Kawashima
Section VI. Functions Revealed by Forced Expression or Gene Knockout
19. Expression of Specific Carbohydrates by Transfection with Carbohydrate Modifying Enzymes
Junya Mitoma and Minoru Fukuda
20. N-Glycan Structure Analysis Using Lectins and an α-Mannosidase Activity Assay
Tomoya O. Akama and Michiko N. Fukuda
21. Targeted Disruption of the Gene Encoding Core 1 β1-3-Galactosyltransferase (T-Synthase) Causes Embryonic Lethality and Defective Angiogenesis in Mice
Lijun Xia and Rodger P. McEver
22. Roles of Mucin-Type O-Glycans Synthesized by Core2β1,6-N-Acetylglucosaminyltransferase
Minoru Fukuda
23. Analysis of Leukocyte Rolling In Vivo and In Vitro
Markus Sperandio, John Pickard, Sunil Unnikrishnan, Scott T. Acton, and Klaus Ley
24. Cell Type–Specific Roles of Carbohydrates in Tumor Metastasis
Shihao Chen and Minoru Fukuda
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