Genomics Proteomics and Vaccines 1st Edition by Guido Grandi ISBN 978-0470856161 0470856165

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Product details: 

ISBN 10:  0470856165

ISBN 13: 978-0470856161

Author: Guido Grandi 

While the sequence of the human genome sequence has hit the headlines, extensive exploitation of this for practical applications is still to come. Genomic and post-genomic technologies applied to viral and bacterial pathogens, which are almost equally important from a scientific perspective, have the potential to be translated into useful products and processes much more rapidly.

Genomics, Proteomics and Vaccines introduces the history of vaccinology and discusses how vaccines are expected to evolve in the future. It describes the relevant technologies, including genome sequencing and analysis, DNA microarrays, 2D electrophoresis and 2D chromatography, mass spectrometry and high-throughput protein expression and purification. The book also features examples of the exploitation of genomics and post-genomics in vaccine discovery, and contains useful descriptions of the biology and pathogenesis of clinically important bacterial pathogens.

This book should be of interest to all those working in vaccine discovery and development in pharmaceutical and biotechnology companies as well as in academic institutions

Table of contents: 

PART 1: INTRODUCTION

1 Vaccination: Past, Present and Future

Maria Lattanzi and Rino Rappuoli

1.1 Introduction

1.2 Vaccination: the past

1.3 Vaccination: the present

1.4 Vaccination: the future

1.5 Conclusion: the intangible value of vaccination References

2 Bioinformatics, DNA Microarrays and Proteomics in Vaccine Discovery: Competing or Complementary Technologies?

Guido Grandi

2.1 Introduction

2.2 From genome sequence to vaccine discovery

2.3 A case study: the anti-meningococcus B vaccine

2.4 Comparison of the three approaches

2.5 Conclusions: a ‘nomics’ approach to vaccine discovery References

PART 2: TECHNOLOGIES

3 Genome Sequencing and Analysis

3.1 Hervé Tettelin and Tamara Feldblyum

Introduction 

3.2 Genome sequencing

3.3 Genome analysis

3.4 Conclusion References

4 Understanding DNA Microarrays: Sources and Magnitudes of Variances in DNA Microarray Data Sets

She-pin Hung, Suman Sundaresh, Pierre F. Baldi and G. Wesley Hatfield

4.1 Introduction

4.2 DNA array formats

4.3 Data analysis methods

4.4 Sources and magnitudes of noise in DNA microarray experiments

4.5 Conclusions Acknowledgements References

5 The Proteome, Anno Domini Two Zero Zero Three

Pier Giorgio Righetti, Mahmoud Hamdan, Frederic Reymond and Joël S. Rossier

5.1 Introduction

5.2 Some definitions

5.3 What methods exist to tackle the proteome complexity?

5.4 Quantitative proteomics

5.5 Pre-fractionation in proteome analysis

5.6 Multi-dimensional chromatography

5.7 Protein chip arrays

5.8 Imaging mass spectrometry

Acknowledgements References

6 Mass Spectrometry in Proteomics

Pierre-Alain Binz

6.1 Introduction

6.2 MS technology

6.3 Principle of protein identification based on MS data

6.4 Proteomics workflows References

7 High Throughput Cloning, Expression and Purification Technologies

7.1 Andreas Kreusch and Scott A. Lesley

Introduction 

Gene cloning

7.2 Protein expression 

7.4 High-throughput protein purification

7.5 Validation of the pipeline and outlook

7.6 Conclusion References

PART 3: APPLICATIONS

8 Meningococcus B: from Genome to Vaccine

Davide Serruto, Rino Rappuoli, and Mariagrazia Pizza

8.1 Meningococcus, a major cause of bacterial meningitis

8.2 Group B meningococcus as an example of reverse vaccinology

8.3 Conclusions References

9 Vaccines Against Pathogenic Streptococci

John L. Telford, Immaculada Margarit y Ros, Domenico Maione, Vega Masignani, Hervé Tettelin, Giuliano Bensi, and Guido Grandi

9.1 Introduction

9.2 Comparative genomics of streptococci

9.3 A vaccine against group B streptococcus

9.4 A vaccine against group A streptococcus

9.5 Conclusions References

10 Identification of the ‘Antigenome’ a Novel Tool for Design and Development of Subunit Vaccines Against Bacterial Pathogens

Eszter Nagy, Tamás Henics, Alexander von Gabain and Andreas Meinke

10.1 Introduction

10.2 Small DNA insert libraries a tool to cover a pathogen’s ‘antigenome

10.3 Proper display platforms

10.4 Selected human sera to provide imprints of pathogen encounters

10.5 Cognate antibodies reveal the ‘antigenome’ of a pathogen

10.6 How to retrieve from the ‘antigenome’ the candidate antigens for vaccine development

10.7 Summary and discussion

References

11 Searching the Chlamydia Genomes for New Vaccine Candidates

Giulio Ratti, Oretta Finco and Guido Grandi

11.1 Old problems and new perspectives for chlamydial vaccines

11.2 Post-genomic approaches

11.3 Genomic screening results

11.4 Concluding considerations

2 References

12 Proteomics and Anti-Chlamydia Vaccine Discovery

Gunna Christiansen, Svend Birkelund, Brian B. Vandahl and Allan C Shaw

12.1 Introduction

12.2 Proteome analysis

12.3 Proteomics as a complement for genomics

12.4 Benefits that proteomics provide for vaccine development

References

13 Proteome Analysis of Outer Membrane and Extracellular Proteins from Pseudomonas aeruginosa for Vaccine Discovery

Stuart J. Cordwell and Amanda S. Nouwens

13.2 Membrane proteins in P. aeruginosa

13.3 Extracellular proteins in P. aeruginosa

13.4 Immunogenic proteins and vaccine discovery

13.5 Conclusions

References

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Tags: Guido Grandi, Genomics Proteomics