Genomic Imprinting Methods and Protocols 1st Edition by Wendy Dean, Gavin Kelsey, Wolf Reik, Andrew Ward ISBN 978-1592592111 1592592111

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ISBN 10:   1592592111 

ISBN 13: 978-1592592111

Author: Wendy L. Dean, Gavin Kelsey, Wolf Reik, Andrew Ward

Genomic imprinting is the process by which gene activity is regulated according to parent of origin. Usually, this means that either the maternally inherited or the paternally inherited allele of a gene is expressed while the opposite allele is repressed. The phenomenon is largely restricted to mammals and flowering plants and was first recognized at the level of whole genomes. Nuclear transplantation experiments carried out in mice in the late 1970s established the non-equivalence of the maternal and paternal genomes in mammals, and a similar conclusion was drawn from studies of interploidy crosses of flowering plants that extend back to at least the 1930s. Further mouse genetic studies, involving animals carrying balanced translocations (reviewed in Chapter 3), indicated that imprinted genes were likely to be widely scattered and would form a minority within the mammalian genome. The first imprinted genes were identified in the early 1990s; over forty are now known in mammals and the list continues steadily to expand.

Table of contents: 

1. Generation of Monoparental Embryos for Investigation into Genomic Imprinting – Wendy L. Dean, Gavin Kelsey, Wolf Reik
2. Deriving and Propagating Mouse Embryonic Stem Cell Lines for Studying Genomic Imprinting – Jeffrey R. Mann
3. Balanced Translocations for the Analysis of Imprinted Regions of the Mouse Genome – Anne C. Ferguson-Smith, Maxine Tevendale, Pantelis Georgiades, Valerie Grandjean
4. Production of YAC Transgenic Mice by Pronuclear Injection – Justin F.-X. Ainscough, Rosalind M. John, Sheila C. Barton
5. A Transgenic Approach to Studying Imprinted Genes – Rosalind M. John, Justin F.-X. Ainscough, Sheila C. Barton
6. Methylation-Sensitive Genome Scanning – Izuho Hatada, Tsunehiro Mukai
7. Subtraction-Hybridization Method for the Identification of Imprinted Genes – Fumitoshi Ishino, Yoshimi Kuroiwa, Naoki Miyoshi, Shin Kobayashi, Takashi Kohda, Tomoko Kaneko-Ishino
8. Identification of Imprinted Loci by Methylation – Rachel J. Smith, Gavin Kelsey
9. Ribonuclease Protection – Joanne L. Thorvaldsen, Marisa S. Bartolomei
10. Quantitative RT-PCR-Based Analysis of Allele-Specific Gene Expression – Judith Singer-Sam, Chunguang Gao
11. Allele-Specific In Situ Hybridization (ASISH) – Rolf Ohlsson, Kristian Svensson, Hengmi Cui, Helena Malmikumpu, Gail Adam
12. RNA-FISH to Analyze Allele-Specific Expression – Giovanna Braidotti
13. Flow Cytometry and FISH to Investigate Allele-Specific Replication Timing and Homologous Association of Imprinted Chromosomes – Janine LaSalle, Marc Lalande
14. Southern Analysis Using Methyl-Sensitive Restriction Enzymes – Tom Moore
15. A PCR-Based Method for Studying DNA Methylation – Mira Ariel
16. Bisulfite-Based Methylation Analysis of Imprinted Genes – Sabine Engemann, Osman El-Maarri, Petra Hajkova, Joachim Oswald, Joern Walter
17. Direct Analysis of Chromosome Methylation – Déborah Bourc’his, Evani Viegas-Péquignot
18. In Vitro Methylation of Predetermined Regions in Recombinant DNA Constructs – Ilse Van den Wyngaert, Roger L. P. Adams, Stefan U. Kass
19. In Vitro Methylation of Specific Regions in Recombinant DNA Constructs by Excision and Religation – Ghislaine Dell, Marika Charalambous, Andrew Ward
20. Detection of Methyl-Sensitive DNA-Binding Proteins with Possible Involvement in the Imprinting Phenomenon – Kerstin Otte, Björn Rozell
21. Probing Chromatin Structure with Nuclease Sensitivity Assays – Richard I. Gregory, Sanjeev Khosla, Robert Feil
22. Examining Histone Acetylation at Specific Genomic Regions – Ji-Fan Hu, Andrew R. Hoffman
23. Purification of the MeCP2/Histone Deacetylase Complex from Xenopus laevis – Peter L. Jones, Paul A. Wade, Alan P. Wolffe
24. Reconstitution of Chromatin In Vitro – Kiyoe Ura, Yasufumi Kaneda
25. Genomic Imprinting in Plants – Rinke Vinkenoog, Melissa Spielman, Sally Adams, Hugh G. Dickinson, Rod J. Scott

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Tags: Wendy Dean, Gavin Kelsey, Wolf Reik, Andrew Ward, Genomic Imprinting, Methods and Protocols