Gene Therapy Protocols Production and In Vivo Applications of Gene Transfer Vectors 3rd Edition by Joseph Le Doux ISBN 978-1588299031 1588299031

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Product details: 

ISBN 10: 1588299031

ISBN 13: 978-1588299031

Author:   Joseph M. Le Doux
In Gene Therapy Protocols, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors (Volume 1) and Design and Characterization of Gene Transfer Vectors (Volume 2). The field of gene therapy has undergone remarkable advances, promising to impact human healthcare significantly in the twenty-first century. Today’s technologies can deliver genetic material safely and effectively to cells to slow or halt the progression of disease, and to help repair or regenerate damaged or lost tissues. In this first volume of Gene Therapy Protocols: Production and In Vivo Applications of Gene Transfer Vectors, readers will find a comprehensive resource of current and emerging methods for the production of viral and non-viral gene transfer vectors, as well as detailed protocols for critical applications in stem cell biology, cancer, diabetes, HIV and tissue engineering. This first volume of the new and completely revised third edition of Gene Therapy Protocols will prove a necessary tool for graduate students and postdoctoral fellows and invaluable to basic and clinical researchers in both industry and academia.

Table of contents: 

Contributors

1. Preparation and Quantification of Pseudotyped Retroviral Vector Hong Yu and Young Jik Kwon

2. Production of Retroviral Vectors for Clinical Use.. Kenneth Cornetta, Lilith Reeves, and Scott Cross

3. Methods for the Production of Helper-Dependent Adenoviral Vectors.. Donna J. Palmer and Philip Ng

4. Methods for the Production of First Generation Adenoviral Vectors… Donna J. Palmer and Philip Ng

5. Large-Scale Production of Recombinant Adeno-Associated Viral Vectors.. Alejandro Negrete and Robert M. Kotin

6. Construction and Production of Recombinant Herpes Simplex Virus Vectors William F. Goins, David M. Krisky, James B. Wechuck, Shaohua Huang, and Joseph C. Glorioso

7. Plasmid-Based Gene Transfer in Mouse Skeletal Muscle by Electroporation Jonathan D. Schertzer and Gordon S. Lynch

8. Chitosan Nanoparticle-Mediated Gene Transfer. Dongwon Lee and Shyam S. Mohapatra

9. PEG-PEI Copolymers for Oligonucleotide Delivery to Cells and Tissues

Gordon J. Lutz, Shashank R. Sirsi, and Jason H. Williams

10. Non-Viral Gene Delivery with Cationic Liposome-DNA Complexes. Kai K. Ewert, Ayesha Ahmad, Nathan F. Bouxsein, Heather M. Evans, and Cyrus R. Safinya

11. Applications of Lentiviral Vectors in Noninvasive Molecular Imaging

Abhijit De, Shahriar Shah Yaghoubi, and Sanjeev Sam Gambhir

12. Retroviral Modification of Mesenchymal Stem Cells for Gene Therapy of Hemophilia A.

Christopher B. Doering

13. Transduction of Murine Hematopoietic Stem Cells and In Vivo Selection of Gene-Modified Cells.

Lucienne M. Ide, Elisabeth Javazon, and H. Trent Spencer

14. Assessment of CFTR Function after Gene Transfer In Vitro and In Vivo

Uta Griesenbach, Felix M. Munkonge, Stephanie Sumner-Jones, Emma Holder, Stephen N. Smith, A. Christopher Boyd, Deborah R. Gill, Stephen C. Hyde, David Porteous, and Eric W. F. W. Alton; on behalf of the UK Cystic Fibrosis Gene Therapy Consortium

15. Oncolytic Adenoviruses for Cancer Gene Therapy Ta-Chiang Liu, Stephen H. Thorne, and David H. Kirn

16. Design of Trans-Splicing Adeno-Associated Viral Vectors for Duchenne Muscular Dystrophy Gene Therapy Yi Lai, Dejia Li, Yongping Yue, and Dongsheng Duan

17. Prevention of Type 1 Diabetes in NOD Mice by Genetic Engineering of Hematopoietic Stem Cells. Jessamyn Bagley, Chaorui Tian, and John lacomini

18. Lentiviral Vector Delivery of siRNA and shRNA Encoding Genes into Cultured and Primary Hematopoietic Cells..

Mingjie Li and John J. Rossi

19. Nanoparticle-Mediated Gene Delivery to the Lung. Rajagopal Ramesh

20. Retroviral-Mediated Gene Therapy for the Differentiation of Primary Cells into a Mineralizing Osteoblastic Phenotype…

Jennifer E. Phillips and Andres J. Garcia

21. In Vivo siRNA Delivery to the Mouse Hypothalamus Shows a Role of the Co-Chaperone XAP2 in Regulating TRH Transcription.

Stephanie Decherf, Zahra Hassani, and Barbara A. Demeneix

22. Efficient Retroviral Gene Transfer to Epidermal Stem Cells……. Pedro Lei and Stelios T. Andreadis

Indey

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