Gene Targeting and Embryonic Stem Cells 1st Edition by Jim Mcwhir, Alison Thomson ISBN 978-1859963609 1859963609

Gene Targeting and Embryonic Stem Cells 1st Edition by Jim Mcwhir, Alison Thomson  – Ebook PDF Instant Download/Delivery:  978-1859963609, 1859963609
Full download Gene Targeting and Embryonic Stem Cells 1st Edition after payment

Product details: 

ISBN 10: 1859963609

ISBN 13:  978-1859963609

Author:  Jim Mcwhir, Alison Thomson

Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference. The recent isolation of human embryonic stem cells and the potential of these cells for therapeutic applications has generated an entirely new methodology. Similarly, gene targeting methodology has recently been extended to nuclear donor cells in ungulate species. This volume will be invaluable for both new and established researchers in the field of human embryonic stem cells, and to biotech companies engaged in the production of transgenic proteins in livestock, xenotransplantation and the development of animal models.

Table of contents: 

An Overview of Gene Targeting and Stem Cells

1. An Overview of Gene Targeting and Stem Cells
2. Advances in Gene Targeting in Murine Embryonic Stem Cells
3. Nuclear Transfer with Murine ES Cells

Gene Targeting and Related Techniques

4. Gene Targeting in Sheep
5. RNA Interference

Human Embryonic Stem Cells and Their Applications

6. Human Embryonal Carcinoma Cells: Surrogate Tools in the Study of Human Embryonic Stem Cells
7. Isolation of Human ES Cells
8. In Vitro Culture and Differentiation of Human Embryonic Stem Cells
9. Transfection of Human Embryonic Stem Cells

People also search for:

    
gene targeting and embryonic
    
gene targeting in mice
    
gene expression embryonic development
    
genetic targeting
    
gene targeting definition

Tags: Jim Mcwhir, Alison Thomson, Gene Targeting, Embryonic Stem