Gene Regulation Methods and Protocols 1st Edition by Minou Bina ISBN 978-1627032841 1627032841
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Product details:
ISBN 10: 1627032841
ISBN 13: 978-1627032841
Author: Minou Bina
In this volume of Methods in Molecular Biology™, expert investigators offer comprehensive, complementary, and cutting-edge technologies for studies of gene regulation. The chapters of Gene Regulation: Methods and Protocols are organized to provide an integrated and a coherent view of control systems and their associated components. The protocols are broad in their scope. They include molecular, biochemical, spectroscopic techniques as well as high throughput strategies. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Comprehensive and broad in their scope, the protocols are useful to researchers in many disciplines including molecular biology, genomics, biochemistry, biomedicine, nutrition, and agricultural sciences.
Table of contents:
Gene Regulation
-
Gene Regulation
Minou Bina -
Isolation of Nuclei for Use in Genome-Wide DNase Hypersensitivity Assays to Probe Chromatin Structure
Guoyu Ling, David J. Waxman -
DNase I Digestion of Isolated Nuclei for Genome-Wide Mapping of DNase Hypersensitivity Sites in Chromatin
Guoyu Ling, David J. Waxman -
Isolation and Analysis of DNA Derived from Nucleosome-Free Regions
Matthew Murtha, Yatong Wang, Claudio Basilico, Lisa Dailey -
Acquisition of High Quality DNA for Massive Parallel Sequencing by In Vivo Chromatin Immunoprecipitation
M. van den Boogaard, L. Y. E. Wong, V. M. Christoffels, P. Barnett -
Luciferase Assay to Study the Activity of a Cloned Promoter DNA Fragment
Nina Solberg, Stefan Krauss -
Promoter Deletion Analysis Using a Dual-Luciferase Reporter System
Yong Zhong Xu, Cynthia Kanagaratham, Sylwia Jancik, Danuta Radzioch -
Application of mRNA Display for In Vitro Selection of DNA-Binding Transcription Factor Complexes
Seiji Tateyama, Hiroshi Yanagawa -
Isolation of Intracellular Protein–DNA Complexes Using HaloCHIP, an Antibody-Free Alternative to Chromatin Immunoprecipitation
Danette L. Daniels, Marjeta Urh -
A Modified Yeast One-Hybrid System for Genome-Wide Identification of Transcription Factor Binding Sites
Kazuyuki Yanai -
Identifying Specific Protein–DNA Interactions Using SILAC-Based Quantitative Proteomics
Cornelia G. Spruijt, H. Irem Baymaz, Michiel Vermeulen -
Electrophoretic Mobility-Shift and Super-Shift Assays for Studies and Characterization of Protein–DNA Complexes
Elsie I. Parés-Matos -
Combination of Native and Denaturing PAGE for the Detection of Protein Binding Regions in Long Fragments of Genomic DNA
Kristel Kaer, Mart Speek -
Quantitative NanoProteomics Approach for Protein Complex (QNanoPX) Using Gold Nanoparticle-Based DNA Probe
Shu-Hui Chen, Mei-Yin Lin -
Chromatin Assembly and In Vitro Transcription Analyses for Evaluation of Individual Protein Activities in Multicomponent Transcriptional Complexes
Takayuki Furumatsu, Hiroshi Asahara -
Using FRET to Monitor Protein-Induced DNA Bending: The TBP-TATA Complex as a Model System
Rebecca H. Blair, James A. Goodrich, Jennifer F. Kugel -
Promoter Independent Abortive Transcription Assays Unravel Functional Interactions Between TFIIB and RNA Polymerase
Simone C. Wiesler, Finn Werner, Robert O. J. Weinzierl -
Preparation of Cell Lines for Single-Cell Analysis of Transcriptional Activation Dynamics
Ilona U. Rafalska-Metcalf, Susan M. Janicki -
Peptide Microarrays for Profiling of Serine/Threonine Kinase Activity of Recombinant Kinases and Lysates of Cells and Tissue Samples
Riet Hilhorst, Liesbeth Houkes, Monique Mommersteeg, Joyce Musch, Adriënne van den Berg, Rob Ruijtenbeek -
Immunoaffinity Purification of Protein Complexes from Mammalian Cells
Chieri Tomomori-Sato, Shigeo Sato, Ronald C. Conaway, Joan W. Conaway -
Simple and Efficient Identification of Chromatin Modifying Complexes and Characterization of Complex Composition
Jeong-Heon Lee, David Skalnik -
Heavy Methyl-SILAC Labeling Coupled with Liquid Chromatography and High-Resolution Mass Spectrometry to Study the Dynamics of Site-Specific Histone Methylation
Xing-Jun Cao, Barry M. Zee, Benjamin A. Garcia -
Analysis of p300 Occupancy at the Early Stage of Stem Cell Differentiation by Chromatin Immunoprecipitation
Melanie Le May, Qiao Li -
Mammalian Two-Hybrid Assays for Studies of Interaction of p300 with Transcription Factors
Daniela B. Mendonça, Gustavo Mendonça, Lyndon F. Cooper -
Fluorescence Anisotropy Microplate Assay to Investigate the Interaction of Full-Length Steroid Receptor Coactivator-1a with Steroid Receptors
Chen Zhang, Steven K. Nordeen, David J. Shapiro -
Use of Histone Deacetylase Inhibitors to Examine the Roles of Bromodomain and Histone Acetylation in p300-Dependent Gene Expression
Jihong Chen, Qiao Li -
Histone Deacetylase Inhibitor Valproic Acid as a Small Molecule Inducer to Direct the Differentiation of Pluripotent Stem Cells
Jihong Chen, Natascha Lacroix, Qiao Li -
Sedimentation and Immunoprecipitation Assays for Analyzing Complexes that Repress Transcription
Ping Lu, Bruce S. Hostager, Paul B. Rothman, John D. Colgan -
Methods for Studies of Protein Interactions with Different DNA Methyltransferases
Jianchang Yang
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