Fungal Genomics Methods and Protocols 1st Edition by Manfred Grabherr, Evan Mauceli, Li Jun Ma, Jin Rong Xu, Burton Bluhm ISBN 978-1617790409 1617790409

Fungal Genomics Methods and Protocols 1st Edition by Manfred G. Grabherr, Evan Mauceli, Li-Jun Ma, Jin-Rong Xu, Burton H. Bluhm  – Ebook PDF Instant Download/Delivery: 978-1617790409, 1617790409
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Product details: 

ISBN 10: 1617790409

ISBN 13: 978-1617790409

Author: Manfred G. Grabherr, Evan Mauceli, Li-Jun Ma, Jin-Rong Xu, Burton H. Bluhm 

Having experienced unprecedented growth since the turn of the millennium, the dramatic expansion of resources and techniques in fungal genomics is poised to fundamentally redefine the study of fungal biology.  In Fungal Genomics: Methods and Protocols, expert researchers explore the three most likely fronts upon which the field will advance: the sequencing of more and more fungal genomes, the mining of sequenced genomes for useful information, and most importantly, the use of genomics sequences to provide a foundation for powerful techniques to explain biological processes.  Much of the book is dedicated to explaining established and emerging genomics-based technologies in filamentous fungi, including gene expression profiling techniques, techniques for fungal proteomics as well as various case studies that could be adapted to a wide range of fungi.  Written in the highly successful Methods in Molecular Biology™ series format, protocol chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key unpublished tips, potential pitfalls, common mistakes, and special considerations based on the unique experiences of the contributors.

Authoritative and cutting-edge, Fungal Genomics: Methods and Protocols provides fungal biologists at any stage of their careers a user-friendly resource for fungal genomics, especially as readers branch out into unfamiliar but exciting new areas of study.

Table of contents: 

1. Front Matter

2. Genome Sequencing and Assembly – Manfred G. Grabherr, Evan Mauceli, Li-Jun Ma

3. Targeted Cloning of Fungal Telomeres – Mark L. Farman

4. Identification and Annotation of Repetitive Sequences in Fungal Genomes – Braham Dhillon, Stephen B. Goodwin

5. Next-Generation Sequencing and Potential Applications in Fungal Genomics – Phillip SanMiguel

6. Getting the Most Out of Your Fungal Microarray Data: Two Cost- and Time-Effective Methods – Sandra M. Mathioni, André Beló, Jeffrey P. Townsend, Nicole M. Donofrio

7. Fusarium graminearum from Expression Analysis to Functional Assays – Heather E. Hallen-Adams, Brad L. Cavinder, Frances Trail

8. EST Analysis Pipeline: Use of Distributed Computing Resources – Francisco Javier González, Juan Antonio Vizcaíno

9. The Application of ChIP-chip Analysis in the Rice Blast Pathogen – Soonok Kim, Thomas K. Mitchell

10. Proteome Studies of Filamentous Fungi – Scott E. Baker, Ellen A. Panisko

11. A Bioinformatics Pipeline for Sequence-Based Analyses of Fungal Biodiversity – D. Lee Taylor, Shawn Houston

12. Identifying Protein Complexes by Affinity Purification and Mass Spectrometry Analysis in the Rice Blast Fungus – Wende Liu, Anton Iliuk, Andy Tao, Shengli Ding

13. Large Scale Identification of Genes Involved in Plant–Fungal Interactions Using Illumina’s Sequencing-by-Synthesis Technology – R. C. Venu, Yuan Zhang, Brian Weaver, Peter Carswell, Thomas K. Mitchell, Blake C. Meyers et al.

14. High-Throughput Production of Gene Replacement Mutants in Neurospora crassa – Gyungsoon Park, Hildur V. Colot, Patrick D. Collopy, Svetlana Krystofova, Christopher Crew, Carol Ringelberg et al.

15. Phenotypic Analysis of Neurospora crassa Gene Deletion Strains – Gloria E. Turner

16. Efficient Approaches for Generating GFP Fusion and Epitope-Tagging Constructs in Filamentous Fungi – Xiaoying Zhou, Guotian Li, Jin-Rong Xu

17. Large-Scale Insertional Mutagenesis in Magnaporthe oryzae by Agrobacterium tumefaciens-Mediated Transformation – Xiao-Lin Chen, Jun Yang, You-Liang Peng

18. Molecular Methods for Studying the Cryphonectria parasitica – Hypovirus Experimental System – Angus L. Dawe, Rong Mu, Gloricelys Rivera, Joanna A. Salamon

19. Metabolic Fingerprinting in Fusarium verticillioides to Determine Gene Function – Jonathon E. Smith, Burton H. Bluhm

20. Tapping Genomics to Unravel Ectomycorrhizal Symbiosis – Jonathan M. Plett, Barbara Montanini, Annegret Kohler, Simone Ottonello, Francis Martin

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Tags: Manfred Grabherr, Evan Mauceli, Li Jun Ma, Jin Rong Xu, Burton Bluhm, Fungal Genomics, Methods and Protocols