Food Borne Pathogens Methods and Protocols 1st Edition by Rosabel Falcón, Tatiana d Albuquerque, Maria das Graças de Luna ISBN 978-1592599905 1592599905

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Product details: 

ISBN 10:  1592599905

ISBN 13: 978-1592599905

Author:  Rosabel Falcón, Tatiana d’Albuquerque, Maria das Graças de Luna 

A collection of readily reproducible classic and emerging molecular methods for the laboratory isolation and identification of the pathogens, viruses, and parasites that cause food-borne disease. Among the pathogens covered are specific bacteria, including Salmonella spp, Campylobacter spp., Listeria spp., and Bacillus spp.; viruses, including noroviruses and enteroviruses; and parasites, including Cryptosporidium and seafood nematode worms. The protocols follow the successful Methods in Biotechnology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Table of contents: 

Bacteria

• Detection of Hemolysins in Aeromonas spp. Isolates From Food Sources

• Detection and Purification of Bacillus cereus Enterotoxins

• Campylobacter

• Detection of Clostridium botulinum by Multiplex PCR in Foods and Feces

• Multiplex PCR for Specific Identification of Enterohemorrhagic Escherichia coli Strains in the O157:H7 Complex

• PulseNet’s Step-by-Step Laboratory Protocol for Molecular Subtyping of Listeria monocytogenes by Macrorestriction and Pulsed-Field Gel Electrophoresis

• Plesiomonas shigelloides

• Pulsed-Field Gel Electrophoresis as a Molecular Technique in Salmonella Epidemiological Studies

• Kits for Detection of Food Poisoning Toxins Produced by Bacillus cereus and Staphylococcus aureus

• Microbiological and Molecular Methods to Identify and Characterize Toxigenic Vibrio cholerae From Food Samples

• HPLC Measurement of Aflatoxin B1 and Metabolites in Isolated Rat Hepatocytes

Viruses

• Detection of Noroviruses of Genogroups I and II in Drinking Water by Real-Time One-Step RT-PCR

• Detection of Enteroviruses

• Detection of Hepatitis A Virus and Rotavirus Using Nucleic Acid Sequence-Based Amplification

Parasites

• Isolation and Characterization of Cathepsin-L1 Protease From Fasciola hepatica Excretory-Secretory Products for Serodiagnosis of Human Fasciolosis

• Molecular Biology Methods for Detection and Identification of Cryptosporidium Species in Feces, Water, and Shellfish

• Molecular Identification of Nematode Worms From Seafood (Anisakis spp. and Pseudoterranova spp.) and Meat (Trichinella spp.)
  Giuseppe La Rosa, Stefano D’Amelio, Edoardo Pozio

Parallel Studies to the Analysis of Food-Borne Pathogens

• Approaches to Developing Quantitative Risk Assessment Models
  Enda J. Cummins

• A Review of Surveillance Networks of Food-Borne Diseases
  Camelia Molnar, Rita Wels, Catherine C. Adley

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Tags: Rosabel Falcón, Tatiana d Albuquerque, Maria das Graças de Luna, Food Borne Pathogens, Methods and Protocols