Epidermal Cells Methods and Protocols 1st Edition by Kursad Turksen ISBN 9781592598304 1592598307

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Product details: 

ISBN 10:    1592598307

ISBN 13: 9781592598304

Author: Kursad Turksen

A collection of cutting-edge methods to analyze and manipulate epidermal cellprecursors and mature epidermal cells. These protocols cover different methods and models for culturing epidermal cells, for enriching very early epidermal progenitors, and for studying epidermal cell commitment and differentiation both in vitro and in vivo. Topics of special interest include the derivation, characterization, and utility of epidermal stem cells, mature epidermal cells and their characterization, and applications in regenerative medicine. These readily reproducible techniques broaden our understanding of the biology of epidermal cells and of their utility in normal tissue homeostasis and regenerative medicine applications.

Table of contents: 

Analysis of Epidermal Differentiation

1. Analysis of E2F Factors During Epidermal Differentiation – Wing Y. Chang, Lina Dagnino

2. Analysis of HOX Homeodomain Proteins and Gene Transcripts in the Epidermis – László G. Kömüves, Corey Largman

3. Apoptosis in the Epidermis – Kiyofumi Yamanishi, Chun-Shen Shen, Hitoshi Mizutani

4. Fate of Desmosomal Proteins in Apoptotic Epidermal Cells – Jörg Weiske, Otmar Huber

5. Analysis of Connexin 43 Expression on Keratinocytes Using Flow Cytometry – Maja Matic, Christopher Pullis, Marc G. Golightly, Sanford R. Simon

6. MMP-9 and TIMP-1 Assays in Keratinocyte Cultures – Takashi Kobayashi

7. Characterization of Epithelial Cells in the Hair Follicle With S100 Proteins – Kenji Kizawa, Mayumi Ito

8. Immunoelectron Microscopic Analysis of Cornified Cell Envelopes and Antigen Retrieval – Akemi Ishida-Yamamoto

Analysis of Epidermal Differentiation

1. Analysis of E2F Factors During Epidermal Differentiation – Wing Y. Chang, Lina Dagnino

2. Analysis of HOX Homeodomain Proteins and Gene Transcripts in the Epidermis – László G. Kömüves, Corey Largman

3. Apoptosis in the Epidermis – Kiyofumi Yamanishi, Chun-Shen Shen, Hitoshi Mizutani

4. Fate of Desmosomal Proteins in Apoptotic Epidermal Cells – Jörg Weiske, Otmar Huber

5. Analysis of Connexin 43 Expression on Keratinocytes Using Flow Cytometry – Maja Matic, Christopher Pullis, Marc G. Golightly, Sanford R. Simon

6. MMP-9 and TIMP-1 Assays in Keratinocyte Cultures – Takashi Kobayashi

7. Characterization of Epithelial Cells in the Hair Follicle With S100 Proteins – Kenji Kizawa, Mayumi Ito

8. Immunoelectron Microscopic Analysis of Cornified Cell Envelopes and Antigen Retrieval – Akemi Ishida-Yamamoto

Methods and Approaches for the Analysis for Epidermal Function

9. Front Matter

10. Cell Kinetic Analysis in Artificial Skin Using Immunochemical Methods – Andrea Casasco, Antonia Icaro Cornaglia, Federica Riva, Marco Casasco, Alberto Calligaro

11. Proliferation, Differentiation, and Inflammation in Normal and Hyperproliferative Skin Using Multiparameter Flow Cytometry – Piet E. J. van Erp

12. Fluorimetric DNA Assay of Cell Number – William R. Otto

13. Keratinocyte Transient Transfections – Anthony M. Flores, Brian J. Aneskievich

14. Tetracycline-Regulated Gene Expression in Epidermal Keratinocytes – Richard B. Presland, Philip Fleckman

15. Gene Targeting by Oligonucleotides in Keratinocytes – Olga Igoucheva, Kyonggeun Yoon

16. Promoter Analysis in the Human SPRR Gene Family – David F. Fischer, Claude Backendorf

17. Stable Integration of Large PAC Constructs in Keratinocytes – Sarah H. Williams, Alain Hovnanian

18. Targeted Somatic Mutagenesis in the Mouse Epidermis – Daniel Metzger, Mei Li, Pierre Chambon

19. Methods to Study Protein-Protein Interactions – Jin-Jun Meng, Meghan Rojas, Willis Bacon, John T. Stickney, Wallace Ip

20. Isolation of Recombinant Phage-Displayed Antibodies Recognizing Skin Keratinocytes – Kim Bak Jensen, Peter Kristensen

9. Front Matter

10. Cell Kinetic Analysis in Artificial Skin Using Immunochemical Methods – Andrea Casasco, Antonia Icaro Cornaglia, Federica Riva, Marco Casasco, Alberto Calligaro

11. Proliferation, Differentiation, and Inflammation in Normal and Hyperproliferative Skin Using Multiparameter Flow Cytometry – Piet E. J. van Erp

12. Fluorimetric DNA Assay of Cell Number – William R. Otto

13. Keratinocyte Transient Transfections – Anthony M. Flores, Brian J. Aneskievich

14. Tetracycline-Regulated Gene Expression in Epidermal Keratinocytes – Richard B. Presland, Philip Fleckman

15. Gene Targeting by Oligonucleotides in Keratinocytes – Olga Igoucheva, Kyonggeun Yoon

16. Promoter Analysis in the Human SPRR Gene Family – David F. Fischer, Claude Backendorf

17. Stable Integration of Large PAC Constructs in Keratinocytes – Sarah H. Williams, Alain Hovnanian

18. Targeted Somatic Mutagenesis in the Mouse Epidermis – Daniel Metzger, Mei Li, Pierre Chambon

19. Methods to Study Protein-Protein Interactions – Jin-Jun Meng, Meghan Rojas, Willis Bacon, John T. Stickney, Wallace Ip

20. Isolation of Recombinant Phage-Displayed Antibodies Recognizing Skin Keratinocytes – Kim Bak Jensen, Peter Kristensen

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Tags: Kursad Turksen, Epidermal Cells, Methods and Protocols