Embryonic Stem Cell Protocols Volume 2 Differentiation Models 2nd Edition by Takashi Nakayama, Kursad Turksen, Nobuo Inoue ISBN 9781588297846 1588297846

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Product details: 

ISBN 10: 1588297846 

ISBN 13: 9781588297846  

Author: Takashi Nakayama, Kursad Turksen, Nobuo Inoue

Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume two, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. The first volume, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. A companion CD provides electronic color versions of all illustrations in the book. Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.

Table of contents: 

1. Neural Stem Sphere Method: Induction of Neural Stem Cells and Neurons by Astrocyte-Derived Factors in Embryonic Stem Cells In Vitro
2. Generation and Characterization of Oligodendrocytes From Lineage-Selectable Embryonic Stem Cells In Vitro
3. Derivation and Characterization of Neural Cells From Embryonic Stem Cells Using Nestin Enhancer
4. Optimized Neuronal Differentiation of Murine Embryonic Stem Cells: Role of Cell Density
5. Generation of Inner Ear Cell Types From Embryonic Stem Cells
6. Derivation of Epidermal Colony-Forming Progenitors From Embryonic Stem Cell Cultures
7. Directing Epidermal Fate Selection by a Novel Co-Culture System
8. In Vitro Generation of T Lymphocytes From Embryonic Stem Cells
9. The Role of Hex in Hemangioblast and Hematopoietic Development
10. Generation of Osteoblasts and Chondrocytes From Embryonic Stem Cells
11. Analysis of Embryonic Stem Cell-Derived Osteogenic Cultures
12. Generation of Chondrocytes From Embryonic Stem Cells
13. Derivation and Characterization of Chondrocytes From Embryonic Stem Cells In Vitro
14. Generation and Characterization of Cardiomyocytes Under Serum-Free Conditions
15. Analysis of Arrhythmic Potential of Embryonic Stem Cell-Derived Cardiomyocytes
16. Derivation and Characterization of Alveolar Epithelial Cells From Murine Embryonic Stem Cells In Vitro
17. Derivation and Characterization of Thyrocyte-Like Cells From Embryonic Stem Cells In Vitro
18. Derivation and Characterization of Gut-Like Structures From Embryonic Stem Cells
19. Formation of Gut-Like Structures In Vitro From Mouse Embryonic Stem Cells
20. In Vitro Derivation and Expansion of Endothelial Cells From Embryonic Stem Cells
21. Differentiation of Mouse Embryonic Stem Cells Into Endothelial Cells: Genetic Selection and Potential Use In Vivo
22. Integrins and Vascular Development in Differentiated Embryonic Stem Cells In Vitro
23. TGF-b Signaling in Embryonic Stem Cell-Derived Endothelial Cells
24. The Role of the Adapter Protein SHB in Embryonic Stem Cell Differentiation Into the Pancreatic β-cell and Endothelial Lineages
25. In Vitro Differentiation of Embryonic Stem Cells Into the Pancreatic Lineage
26. Derivation and Characterization of Hepatocytes From Embryonic Stem Cells In Vitro
27. Differentiation of Embryonic Stem Cells to Retinal Cells In Vitro
28. Derivation and Characterization of Lentoid Bodies and Retinal Pigment Epithelial Cells From Monkey Embryonic Stem Cells In Vitro
29. Differentiation of Rhesus Monkey Embryonic Stem Cells in Three-Dimensional Collagen Matrix
30. Index

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Tags: Takashi Nakayama, Kursad Turksen, Nobuo Inoue, Embryonic Stem, Differentiation Models