Differential Display Methods and Protocols 2nd Edition by Shitao Yang – Ebook PDF Instant Download/Delivery: 9781588293381, 1588293386
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Product details:
ISBN 10: 1588293386
ISBN 13: 9781588293381
Author: Shitao Yang
Since the first edition of this book dedicated to differential display (DD) technology was published in 1997, we have witnessed an explosive interest in studying differential gene expression. The gene-hunting euphoria was initially powered by the invention of DD, which was gradually overtaken by DNA microarray technology in recent years. Then why is there still the need for second edition of this DD book? First of all, DD still enjoys a substantial lead over DNA microarrays in the ISI citation data (see Table 1), despite the h- dreds of millions of dollars spent each year on arrays. This may come as a surprise to many, but to us it implies that many of the DNA microarray studies went unpublished owing to their unfulfilled promises (1). Second, unlike DNA microarrays, DD is an “open”-ended gene discovery method that does not depend on prior genome sequence information of the organism being studied. As such, DD is applicable to the study of all living organisms—from bacteria, fungi, insects, fish, plants, to mammals—even when their genomes are not sequenced. Second, DD is more accessible technically and financially to most cost-conscious “cottage-industry” academic laboratories. So clearly DD still has its unique place in the modern molecular biological toolbox for gene expression analysis.
Table of contents:
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Front Matter
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Methodologies
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Global Analysis of Gene Expression by Differential Display
Shitao Yang, Peng Liang -
Automation of Fluorescent Differential Display With Digital Readout
Jonathan D. Meade, Yong-jig Cho, Jeffrey S. Fisher, Jamie C. Walden, Zhen Guo, Peng Liang -
Ordered Differential Display
Mikhail V. Matz, Ella A. Meleshkevitch -
GeneCalling®
Richard A. Shimkets -
High-Density Sampling Differential Display of Prokaryotic mRNAs With RAP-PCR
Dana M. Walters, Pierre E. Rouvière -
Vertical Arrays
Rosana Risques, Gaelle Rondeau, Martin Judex, Michael McClelland, John Welsh -
Automated Pattern Ranking in Differential Display Data Analysis
Tero Aittokallio, Pekka Ojala, Timo J. Nevalainen, Olli S. Nevalainen -
Linking cDNA-AFLP-Based Gene Expression Patterns and ESTs
Ling Qin, Pjotr Prins, Johannes Helder -
Applications
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Differentially Expressed Genes Associated With Hepatitis B Virus HBx and MHBs Protein Function in Hepatocellular Carcinoma
Dae-Ghon Kim -
Identification of Disease Markers by Differential Display
Michael Clinton, Gino Miele, Sunil Nandi, Derek McBride -
Saturation Screening for p53 Target Genes by Digital Fluorescent Differential Display
Yong-jig Cho, Susanne Stein, Roger S. Jackson, Peng Liang -
Identification of p53-Regulated Genes by the Method of Differential Display
Yunping Lin, Roger P. Leng, Samuel Benchimol -
Identification by Differential Display of IL-24 Autocrine Loop Activated by Ras Oncogenes
Zhongjia Tan, Mai Wang, Peng Liang -
Comprehensive Analysis of Ovarian Gene Expression During Ovulation Using Differential Display
Lawrence L. Espey -
Functional Analysis of Nocturnin
Julie E. Baggs, Carla B. Green -
Isolation and Characterization of Anthocyanin 5-O-Glucosyltransferase in Perilla frutescens var. crispa by Differential Display
Mami Yamazaki, Kazuki Saito -
Identification of Target Genes of a Yeast Transcriptional Repressor
Bernard Mai, Linda L. Breeden -
Detection of an mRNA Polymorphism by Differential Display
Shan Liang, S. Paul Rossby, Peng Liang, Richard C. Shelton, D. Hal Manier, Amitabha Chakrabarti et al. -
Silencing in Yeast
Sergey V. Ivanov, Alla V. Ivanova -
Identification of mRNA Bound to RNA Binding Proteins by Differential Display
Anne Carr-Schmid, Xinfu Jiao, Megerditch Kiledjian -
Back Matter
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