Diabetes Mellitus 1st Edition by Sabire Özcan ISBN 978-1588291486 1588291486

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Product details: 

ISBN 10:  1588291486

ISBN 13: 978-1588291486

Author:  Sabire Özcan 

Diabetes mellitus is the collective name for a group of diseases associated with hyperglycemia (high levels of blood glucose) caused by defects in insulin p- duction, insulin action, or both. About 6. 2% of the US population (17 million people) have diabetes mellitus. It is the leading cause of kidney failure, bli- ness, and amputations. It is also a major risk factor for heart diseases, stroke, and birth defects. Diabetes Mellitus: Methods and Protocols provides a state-of-the-art account of the experimental methodology for studying the molecular defects leading to diabetes mellitus, both at the molecular and biochemical levels. The chapters cover a wide range of topics written by experts in their respective fields and are organized in two sections: Insulin Production and Insulin Action. The detailed experimental protocols presented, including the notes of interest, provide a very useful tool for basic researchers and clinicians for investigating and treating this disease. Each chapter starts with an introduction to a specific technique and explains its application in the field of diabetes research. Following the list of materials, a detailed description of the technique is presented in the methods section in a way that enables the successful execution of the protocol. The “Notes” section at the end discusses the pitfalls of the technique and alternative approaches. I am grateful to the numerous scientists who have contributed to this volume by writing both highly detailed and understandable chapters.

Table of contents: 

1. Insulin Production
2. Isolation of Islets of Langerhans from Rodent Pancreas
3. Purification of Rat Pancreatic β-Cells by Fluorescence-Activated Cell Sorting
4. Assessment of Insulin Secretion in the Mouse
5. Detection of Insulin Production by Immunohistochemistry
6. Quantification of the Level of Insulin Gene Expression
7. Chromatin Immunoprecipitation Using Isolated Islets of Langerhans
8. Adenoviral Gene Transfer into β-Cell Lines
9. Utilization of NOD Mice in the Study of Type 1 Diabetes
10. Insulin Action
11. Transfection of DNA into 3T3-L1 Adipocytes by Electroporation
12. Analysis of Insulin-Stimulated Glucose Uptake in Differentiated 3T3-L1 Adipocytes
13. Fractionation Analysis of the Subcellular Distribution of GLUT-4 in 3T3-L1 Adipocytes
14. Visualization and Quantitation of Integral Membrane Proteins Using a Plasma Membrane Sheet Assay
15. Assaying Tyrosine Phosphorylation of Insulin Receptor and Insulin Receptor Substrates
16. Measuring Insulin-Stimulated Phosphatidyl-Inositol 3-Kinase Activity
17. Assaying AKT/Protein Kinase B Activity
18. Measurements of Cellular Phosphoinositide Levels in 3T3-L1 Adipocytes
19. Measurement of Glycogen Synthesis and Glycogen Synthase Activity in 3T3-L1 Adipocytes
20. Measurement of Contraction-Stimulated GLUT-4 Translocation in Isolated Skeletal Muscle
21. Single Embryo Measurement of Basal- and Insulin-Stimulated Glucose Uptake
22. Immunohistologic Staining of Muscle and Embryos to Detect Insulin-Stimulated Translocation of Glucose Transporters

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Tags: Sabire Özcan, Diabetes Mellitus