Confocal Microscopy Methods and Protocols 1st Edition by Stephen Paddock ISBN 978-0896035263 0896035263

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Product details: 

ISBN 10:  0896035263

ISBN 13: 978-0896035263

Author: Stephen W. Paddock 

Stephen Paddock and a highly skilled panel of experts lead the researcher using confocal techniques from the bench top, through the imaging process, to the journal page. They concisely describe all the key stages of confocal imaging-from tissue sampling methods, through the staining process, to the manipulation, presentation, and publication of the realized image. Written in a user-friendly, nontechnical style, the methods specifically cover most of the commonly used model organisms: worms, sea urchins, flies, plants, yeast, frogs, and zebrafish. The powerful hands-on methods collected here will help even the novice to produce first-class cover-quality confocal images.

Table of contents: 

1. An Introduction to Confocal Imaging

2. Practical Considerations for Collecting Confocal Images

3. Fluorescent Probes for Confocal Microscopy

4. Imaging Gene Expression Using Antibody Probes

5. Single and Double FISH Probes for Drosophila

6. Confocal Microscopy of Plant Cells

7. Preparation of Yeast Cells for Confocal Microscopy

8. Confocal Methods for Caenorhabditis elegans

9. Imaging Sea Urchin Fertilization

10. Imaging Immunolabeled Drosophila Embryos by Confocal Microscopy

11. Confocal Microscopy on Xenopus laevis Oocytes and Embryos

12. Analyzing Morphogenetic Cell Behaviors in Vitally Stained Zebrafish Embryos

13. Confocal Imaging of Living Cells in Intact Embryos

14. Live Confocal Analysis with Fluorescently Labeled Proteins

15. Live Imaging with Green Fluorescent Protein

16. Fluorescent Calcium Indicators: Subcellular Behavior and Use in Confocal Imaging

17. Intracellular pH and pCa Measurement

18. Measuring Dynamic Cell Volume In Situ by Confocal Microscopy

19. Imaging Thick Tissues with Confocal Microscopy

20. Measurement in the Confocal Microscope

21. Presentation of Confocal Images

22. The Preparation of Stereoscopic 3D Illustrations of Confocal Data Sets for Publications and Slides

23. Information Management of Confocal Microscopy Images

24. Morphing Confocal Images and Digital Movie Production

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Tags: Stephen Paddock, Confocal Microscopy, Methods and Protocols