Cervical Cancer Methods and Protocols 1st Edition by Daniel Keppler, Athena Lin ISBN 1493952854 978-1493952854
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Product details:
ISBN 10: 1493952854
ISBN 13: 978-1493952854
Author: Daniel Keppler, Athena W. Lin
Representing the most relevant procedures and technologies aiding the advance of the field of HPV-mediated carcinogenesis of the cervix and other anatomical regions of squamocolumnar transition, such as the anorectum, penis, and oropharynx, Cervical Cancer: Methods and Protocols compiles a detailed collection of practical chapters. The first half of the book covers HPV types, pathogenesis of cervical cancer (CxCA), prevention, and novel potential drug targets, while the second half explores pathology, genomics, modeling of CxCA, and experimental therapeutic strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and vital, Cervical Cancer: Methods and Protocols serves as a valuable resource to both bench scientists and clinicians who step into the realm of high-risk HPVs and CxCA for the first time or those who wish to learn novel approaches or expand their toolbox for the study of CxCA.
Table of contents:
1 Approaches to Detecting, Typing, and Staging of CxCA
- Immunocytochemical Analysis of the Cervical Pap Smear
- Diagnosis of HPV-Negative, Gastric-Type Adenocarcinoma of the Endocervix
- Targeting of the HPV-16 E7 Protein by RNA Aptamers
- The Use of MYBL2 as a Novel Candidate Biomarker of Cervical Cancer
- Fixation Methods for the Preservation of Morphology, RNAs, and Proteins
2 Genomic Analyses of CxCA
- Assessment of the HPV DNA Methylation Status in Cervical Lesions
- MeDIP-on-Chip for Methylation Profiling
- Use of DBD-FISH for the Study of Cervical Cancer Progression
3 Cellular Assays and Cell Culture Models of CxCA
- A Quantitative and High-Throughput Assay of HPV DNA Replication
- Native HPV Production, Quantification, and Infectivity Analysis
- Functional Analysis of HPV-Like Particle-Activated Langerhans Cells
- Assessment of the Radiation Sensitivity of Cervical Cancer Cell Lines
4 Animal Models of CxCA and Experimental Therapeutic Strategies
- Mouse Model of Cervicovaginal Papillomavirus Infection
- Establishment of Orthotopic Primary Cervix Cancer Xenografts
- Generation of K14-E7/∆N87βcat Double Transgenic Mice
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Daniel Keppler,Athena Lin,Cervical Cancer,Methods and Protocols