Cell Imaging Techniques Methods and Protocols 2nd Edition by Douglas J. Taatjes, Jürgen Roth- Ebook PDF Instant Download/Delivery: 978-1627030564, 1627030564
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Product details:
ISBN 10: 1627030564
ISBN 13: 978-1627030564
Author: Douglas J. Taatjes, Jürgen Roth
Cell Imaging is rapidly evolving as new technologies and new imaging advances continue to be introduced. In the second edition of Cell Imaging Techniques: Methods and Protocols expands upon the previous editions with current techniques that includes confocal microscopy, transmission electron microscopy, atomic force microscopy, and laser microdissection. With new chapters covering colocalization analysis of fluorescent probes, correlative light and electron microscopy, environmental scanning electron microscopy, light sheet microscopy, intravital microscopy, high throughput microscopy, and stereological techniques. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls
Authoritative and cutting-edge, Cell Imaging Techniques: Methods and Protocols, Second Edition is an easily accessible volume of protocols to be used with a variety of imaging-based equipment likely available in a core imaging facility.
Table of contents:
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Front Matter
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Digital Images Are Data: And Should Be Treated as Such – Douglas W. Cromey
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Epi‑Fluorescence Microscopy – Donna J. Webb, Claire M. Brown
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Live‑Cell Migration and Adhesion Turnover Assays – J. Lacoste, K. Young, Claire M. Brown
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Multifluorescence Confocal Microscopy: Application for a Quantitative Analysis of Hemostatic Proteins in Human Venous Valves – Winifred E. Trotman, Douglas J. Taatjes, Edwin G. Bovill
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Colocalization Analysis in Fluorescence Microscopy – Jeremy Adler, Ingela Parmryd
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A Time‑Lapse Imaging Assay to Study Nuclear Envelope Breakdown – Sunita S. Shankaran, Douglas R. Mackay, Katharine S. Ullman
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Light Sheet Microscopy in Cell Biology – Raju Tomer, Khaled Khairy, Philipp J. Keller
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Image‑Based High‑Throughput Screening for Inhibitors of Angiogenesis – Lasse Evensen, Wolfgang Link, James B. Lorens
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Intravital Microscopy to Image Membrane Trafficking in Live Rats – Andrius Masedunskas, Monika Sramkova, Laura Parente, Roberto Weigert
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Imaging Non‑fluorescent Nanoparticles in Living Cells with Wavelength‑Dependent Differential Interference Contrast Microscopy and Planar Illumination Microscopy – Wei Sun, Lehui Xiao, Ning Fang
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Laser Scanning Cytometry: Principles and Applications—An Update – Piotr Pozarowski, Elena Holden, Zbigniew Darzynkiewicz
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Laser Capture Microdissection for Protein and NanoString RNA Analysis – Yelena Golubeva, Rosalba Salcedo, Claudius Mueller, Lance A. Liotta, Virginia Espina
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Viewing Dynamic Interactions of Proteins and a Model Lipid Membrane with Atomic Force Microscopy – Anthony S. Quinn, Jacob H. Rand, Xiao‑Xuan Wu, Douglas J. Taatjes
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Mica Functionalization for Imaging of DNA and Protein‑DNA Complexes with Atomic Force Microscopy – Luda S. Shlyakhtenko, Alexander A. Gall, Yuri L. Lyubchenko
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Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation – Joanna L. MacKay, Sanjay Kumar
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Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells – Lilia A. Chtcheglova, Peter Hinterdorfer
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Porosome: The Secretory NanoMachine in Cells – Bhanu P. Jena
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Stereology and Morphometry of Lung Tissue – Christian Mühlfeld, Lars Knudsen, Matthias Ochs
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A Novel Combined Imaging/Morphometrical Method for the Analysis of Human Sural Nerve Biopsies for Clinical Diagnosis – Michele A. von Turkovich, Marilyn P. Wadsworth, William W. Pendlebury, Douglas J. Taatjes
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