Cell Free Protein Synthesis 2014th Edition by Kirill Alexandrov, Wayne Johnston ISBN 1627037810 978-1627037815

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Product details: 

ISBN 10: 1627037810

ISBN 13:  978-1627037815

Author: Kirill Alexandrov,  Wayne A. Johnston

Cell-free protein expression promises to narrow the technological gap between DNA and protein technologies and provide a platform for broad application of synthetic biology principles in the Life Sciences. It is a rapid and high throughput methodology for the conversion of DNA encoded genetic information into protein-mediated biochemical activities. Cell-Free Protein Synthesis: Methods and Protocols brings together the key opinion leaders of cell-free technology development and provides case studies and detailed protocols for the application of cell-free methodology. Chapters cover the main directions in the development of cell-free technologies including several recently developed cell-free systems, as well as a number of applications of cell-free systems ranging from discovery of biofuel enzymes to in vitro assembly of viruses. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Table of contents: 

1. Production of Eukaryotic Cell-Free Lysate from Leishmania tarentolae — Wayne A. Johnston, Kirill Alexandrov

2. Bioinformatics Analysis and Optimization of Cell-Free Protein Synthesis — Alexander A. Tokmakov, Atsushi Kurotani, Mikako Shirouzu, Yasuo Fukami, Shigeyuki Yokoyama

3. A Cell-Free Expression Screen to Identify Fusion Tags for Improved Protein Expression — Andrew Kralicek

4. One-Pot, Microscale Cell-Free Enzyme Expression and Screening — Aarthi Chandrasekaran, Anup K. Singh

5. Cell-Free Translation of Biofuel Enzymes — Taichi E. Takasuka, Johnnie A. Walker, Lai F. Bergeman, Kirk A. Vander Meulen, Shin-ichi Makino, Nathaniel L. Elsen et al.

6. Cloning-Independent Expression and Screening of Enzymes Using Cell-Free Protein Synthesis Systems — Yong-Chan Kwon, Jae-Kwang Song, Dong-Myung Kim

7. High-Level Cell-Free Production of Membrane Proteins with Nanodiscs — Christian Roos, Lei Kai, Stefan Haberstock, Davide Proverbio, Umesh Ghoshdastider, Yi Ma et al.

8. Cell-Free Protein-Based Enzyme Discovery and Protein–Ligand Interaction Study — Sabrina Guillemer, Cécile Persillon, Jean-Michel Masson, Gilles Ravot

9. Human Cell Extract-Derived Cell-Free Systems for Virus Synthesis — Tominari Kobayashi, Kodai Machida, Hiroaki Imataka

10. Cell-Free Protein Synthesis in Microfluidic 96-Well Plates — Kirsten Jackson, Ruba Khnouf, Z. Hugh Fan

11. Preparation of Multiple Site-Specific Mutant Proteins for NMR Studies by PCR-Directed Cell-Free Protein Synthesis — Kiyoshi Ozawa, Ruhu Qi

12. Site-Specific Incorporation of Unnatural Amino Acids into Proteins by Cell-Free Protein Synthesis — Kiyoshi Ozawa, Choy Theng Loh

13. In Vitro Translation of Papillomavirus Authentic and Codon-Modified L1 Capsid Gene mRNAs in Mouse Keratinocyte Cell-Free Lysate — Kong-Nan Zhao

14. An Optimized Yeast Cell-Free Lysate System for In Vitro Translation of Human Virus mRNA — Xiao Wang, Liang Zhao, Kong-Nan Zhao

15. In Vitro Translation-Based Protein Kinase Substrate Identification — Szilvia K. Nagy, Tamás Mészáros

16. Preparation of Protein Arrays Using Cell-Free Protein Expression — Elizabeth A. Cook, Mingyue He

17. Posttranscriptional Control of Protein Synthesis in Drosophila S2 Cell-Free System — Motoaki Wakiyama, Shigeyuki Yokoyama

18. Cell-Free Membrane Protein Expression — Tomomi Kimura-Soyema, Mikako Shirouzu, Shigeyuki Yokoyama

19. The PURE System for Protein Production

20. A Cell-Free Protein Synthesis System from Insect Cells — Toru Ezure, Takashi Suzuki, Eiji Ando

21. A Cell-Free Expression Platform for Production of Protein Microarrays — Xristo Zárate, David W. Galbraith

22. Errata

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Tags: Kirill Alexandrov, Wayne Johnston, Cell Free, Protein Synthesis