Cancer Cytogenetics Methods and Protocols 1st Edition by Thomas Wan ISBN 1493967010 978-1493967018

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Product details: 

ISBN 10: 1493967010 

ISBN 13: 978-1493967018

Author: Thomas S.K. Wan

This volume provides readers with detailed protocols covering the main cancer cytogenetics techniques needed for clinical utilization and research purposes. The chapters in this book cover topics such as chromosome preparation for myeloid malignancies; chromosome bandings; fluorescence in situ hybridization probe preparation; array-based comparative genomic hybridization; and cytogenetic nomenclature and reporting. The updated reviews on chromosomal abnormalities in hematological malignancies are excellent guides for cytogenetics data interpretations and specific malignant diseases correlation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Cancer Cytogenetics: Methods and Protocolsis a valuable resource for the novice in cytogenetics because it provides helpful guiding protocols, but it’s also great for those who are already engaged in the field and are looking for some technical hints.

Table of contents: 

1 Cancer Cytogenetics: An Introduction
2 Chromosome Preparation for Myeloid Malignancies
3 Chromosome Preparation for Acute Lymphoblastic Leukemia
4 Chromosome Preparation for Chronic Lymphoid Malignancies
5 Cytogenetic Harvesting of Cancer Cells and Cell Lines
6 Chromosome Bandings
7 Chromosome Recognition
8 Application of Fluorescence In Situ Hybridization Technology in Malignancies
9 Fluorescence In Situ Hybridization Probe Preparation
10 Fluorescence In Situ Hybridization Probe Validation for Clinical Use
11 Fluorescence In Situ Hybridization on Tissue Sections
12 Cytoplasmic Immunoglobulin Light Chain Revelation and Interphase Fluorescence In Situ Hybridization in Myeloma
13 Quantitate Fluorescence In Situ Hybridization (QFISH)
14 High Resolution of Fiber-Fluorescence In Situ Hybridization (Fiber-FISH)
15 Array-Based Comparative Genomic Hybridization (aCGH)
16 Multicolor Karyotyping and Fluorescence In Situ Hybridization-Banding (MCB/mBAND)
17 Cytogenetics for Biological Dosimetry
18 Recurrent Cytogenetic Abnormalities in Myelodysplastic Syndromes
19 Recurrent Cytogenetic Abnormalities in Acute Myeloid Leukemia
20 Recurrent Cytogenetic Abnormalities in Myeloproliferative Neoplasms and Chronic Myeloid Leukemia
21 Recurrent Cytogenetic Abnormalities in Acute Lymphoid Leukemia
22 Recurrent Cytogenetic Abnormalities in Non-Hodgkin’s Lymphoma and Chronic Lymphocytic Leukemia
23 Recurrent Cytogenetic Abnormalities in Multiple Myeloma
24 Cytogenetic Nomenclature and Reporting
25 Cytogenetic Resources and Information

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Thomas Wan,Cancer Cytogenetics,Methods and Protocols