Calcium measurement methods 1st Edition by Alexei Verkhratsky, Ole H. Petersen- Ebook PDF Instant Download/Delivery: 9781607614753, 1607614758
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Product details:
ISBN 10: 1607614758
ISBN 13: 9781607614753
Author: Alexei Verkhratsky, Ole H. Petersen, Alexei Verkhratsky, Ole H. Petersen
Since the introduction of fluorescent calcium indicators and the subsequent development of capacities for real-time monitoring and imaging of calcium movements in the intact cells studied in isolation, in situ and in vivo, the complex and vital calcium signaling system has been illuminated, proving calcium signals to be excellent universal reporters of cellular activity. In “Calcium Measurement Methods”, a team of renowned experts in the field contribute methods dedicated to calcium imaging in neural cells with a balance between the main principles of calcium imaging and specific applications of the technique to neural tissues. The book’s contents show how the combination of different indicators and recently developed microscopic techniques lead to spectacular successes in the imaging of neural cells in the living brain in both physiological and pathophysiological models. As a volume in the successful Neuromethods™ series, the chapters provide authoritative reviews including up-to-date detailed protocols of the most commonly used approaches in the field.
Cutting-edge and concise, “Calcium Measurement Methods” serves as an ideal reference to aid the many scientists worldwide engaged in calcium imaging of the nervous system.
Table of contents:
-
Principles of the Ca²⁺ Homeostatic/Signalling System
Alexei Verkhratsky, Ole H. Petersen -
Ca²⁺ Recordings: Hardware and Software (From Microscopes to Cameras)
Emil C. Toescu, Jeremy Graham -
Ca²⁺ Imaging: Principles of Analysis and Enhancement
Fabio Mammano, Mario Bortolozzi -
Bioluminescent Ca²⁺ Indicators
Laura Fedrizzi, Marisa Brini -
Monitoring Calcium Levels with Genetically Encoded Indicators
Olga Garaschuk, Oliver Griesbeck -
Intracellular Calcium-Sensitive Microelectrodes
Roger C. Thomas -
Ca²⁺ Caging and Uncaging
Shin Hye Kim, Myoung Kyu Park -
Ca²⁺ Imaging of Intracellular Organelles: Endoplasmic Reticulum
Robert Blum, Ole H. Petersen, Alexei Verkhratsky -
Ca²⁺ Imaging of Intracellular Organelles: Mitochondria
Lucía Núñez, Carlos Villalobos, María Teresa Alonso, Javier García-Sancho -
Ca²⁺ Imaging of Dendrites and Spines
Knut Holthoff -
In Vivo Ca²⁺ Imaging of the Living Brain Using Multi-Cell Bolus Loading Technique
Gerhard Eichhoff, Yury Kovalchuk, Zsuzsanna Varga, Alexei Verkhratsky, Olga Garaschuk -
Ca²⁺ Imaging of Glia
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