Artificial Riboswitches Methods and Protocols 1st Edition by Atsushi Ogawa ISBN 978-1493963300 1493963309
Original price was: $50.00.$35.00Current price is: $35.00.
Artificial Riboswitches Methods and Protocols 1st Edition by Atsushi Ogawa – Ebook PDF Instant Download/Delivery: 978-1493963300, 1493963309
Full download Artificial Riboswitches Methods and Protocols 1st Edition after payment
Product details:
ISBN 10: 1493963309
ISBN 13: 978-1493963300
Author: Atsushi Ogawa
Artificial riboswitches and other ligand-responsive gene regulators make it possible to switch protein synthesis ON or OFF with arbitrary ligand molecules. Artificial Riboswitches: Methods and Protocols focuses on the state-of-the-art methods developed in recent years for creating artificial riboswitches, therefore this volume could be regarded as a collection of recipes for the gene circuit elements in synthetic biology and metabolic engineering. Chapters cover topics such as screening or rational design methods for obtaining artificial riboswitches that function in either bacterial or eukaryotic translational systems, protocols for evaluating the activities of the resultant riboswitches, as well as protocols for construction of ligand-dependent, trans-acting gene regulators. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Artificial Riboswitches: Methods and Protocols seeks to serve not only bioengineers who aim to reprogram cell behaviors and molecular biologists who leverage these regulators for genetic studies, but to all researchers interested in this fascinating field.
Table of contents:
1 Computational Design of RNA Libraries for In Vitro Selection of Aptamers Yaroslav G. Chushak, Jennifer A. Martin, Jorge L. Chávez, Nancy Kelley-Loughnane, and Morley O. Stone
2 In Vitro Selection of RNA Aptamers for a Small-Molecule Dye.. Asako Murata and Shin-ichi Sato
3 Development of Photoswitchable RNA Aptamer-Ligand Complexes.. Gosuke Hayashi and Kazuhiko Nakatani
4 Identification of RNA Aptamers Against Recombinant Proteins with a Hexa-Histidine Tag
Shoji Obuchi
5 Artificial Riboswitch Selection: A FACS-Based Approach. Zohaib Ghazi, Casey C. Fowler, and Yingfu Li
6 FRET-Based Optical Assay for Selection of Artificial Riboswitches. Svetlana V. Harbaugh, Molly E. Chapleau, Yaroslav G. Chushak, Morley O. Stone, and Nancy Kelley-Loughnane
7 Portable Two-Way Riboswitches: Design and Engineering Ye Jin and Jian-Dong Huang
8 Generation of Orthogonally Selective Bacterial Riboswitches by Targeted Mutagenesis and In Vivo Screening Helen A. Vincent, Christopher J. Robinson, Ming-Cheng Wu, Neil Dixon, and Jason Micklefield
9 Dual Genetic Selection of Synthetic Riboswitches in Escherichia coli Yoko Nomura and Yohei Yokobayashi
10 Nucleotide Kinase-Based Selection System for Genetic Switches Kohei Ike and Daisuke Umeno
11 Measuring Riboswitch Activity In Vitro and in Artificial Cells with Purified Transcription-Translation Machinery Laura Martini and Sheref S. Mansy
12 Rational Design of Artificial ON-Riboswitches Atsushi Ogawa
13 Engineering Protein-Responsive mRNA Switch in Mammalian Cells……. Kei Endo and Hirohide Saito
14 Guanine-Tethered Antisense Oligonucleotides
as Synthetic Riborcgulators
Masaki Hagihara
15 In Vitro Selection of Allosteric Ribozymes that Sense
the Bacterial Second Messenger c-di-GMP
Kazuhiro Furukawa, Hongzhou Gu, and Ronald R. Breaker
16 Dual-Selection for Evolution of In Vivo Functional Aptazymes
as Riboswitch Parts…
Jonathan A. Goler, James M. Carothers, and Jay D. Keasling
17 In Vivo Screening for Aptazyme-Based Bacterial Riboswitches.
Charlotte Rehm and Jörg S. Hartig
18 Engineered Riboswitch as a Gene-Regulatory Platform
for Reducing Antibiotic Resistance
Libing Liu and Shu Wang
19 Construction of Ligand-Responsive MicroRNAs that Operate Through Inhibition of Drosha Processing.
Chase L. Beisel, Ryan J. Bloom, and Christina D. Smolke
20 A Three-Dimensional Design Strategy for a Protein-Responsive
shRNA Switch
Shunnichi Kashida and Hirohide Saito
Index
People also search for:
5 business laws
regulation x is also known as what law
business law and regulation reviewer
business law and regulation reviewer pdf
business law reg reddit
Tags: Atsushi Ogawa, Artificial Riboswitches, Methods and Protocols