Animal Endo SiRNAs Methods and Protocols 1st Edition by Andreas Werner ISBN 978-1493941605 1493941607
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Product details:
ISBN 10: 1493941607
ISBN 13: 978-1493941605
Author: Andreas Werner
Animal Endo-SiRNAs: Methods and Protocols presents a variety of approaches to investigate endo-siRNAs. These include protocols applicable to study short RNAs expressed at a low level and model systems that are particularly suitable to investigate specific aspects of endo-siRNAs, their synthesis, their genomics or regulatory role. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Animal Endo-SiRNAs: Methods and Protocols contains practical tips that are absent in standard lab manuals.
Table of contents:
1 Targeted Small Noncoding RNA-Directed Gene Activation in Human Cells. Caio Damski and Kevin V. Morris
2 Isolation of Chromatoid Bodies from Mouse Testis as a Rich Source of Short RNAs. Oliver Meikar and Noora Kotaja
3 Generation of Endo-siRNAs in Xenopus laevis Oocytes Sammer Alnumeir and Andreas Werner
4 Analysis of Endo-siRNAs in Drosophila. Katharina Elmer, Stephanie Helfer, Milijana Mirkovic-Hösle, and Klaus Förstemann
5 Methods for Studying the Biological Consequences of Endo-siRNA Deficiency in Drosophila melanogaster. Do-Hwan Lim, Chun-Tack Oh, Sung-Jun Han, and Young Sik Lee
6 Small RNA Library Cloning Procedure for Deep Sequencing of Specific Endogenous siRNA Classes in Caenorhabditis elegans. Maria C. Ow, Nelson C. Lau, and Sarah E. Hall
7 Assays for Direct and Indirect Effects of C. elegans Endo-siRNAs Philip K. Shin, Jimmy J. Zhuang, and Craig P. Hunter
8 Extraction and Nonradioactive Detection of Small RNA Molecules. Mark Carlile and Andreas Werner
9 p19-Mediated Enrichment and Detection of siRNAs. Jingmin Jin, Larry A. McReynolds, and Monika Gullerova
10 Detection of Small Noncoding RNAs by In Situ Hybridization Using Probes of 2′-O-Methyl RNA+LNA Martin Jensen Søe, Martin Dufva, and Kim Holmstrøm
11 Enhanced Detection of Small RNAs Using a Nonradioactive Approach….. Teresa T. Liu, Zhibua Li, and Bino John
12 Computing siRNA and piRNA Overlap Signatures Christophe Antoniewski
13 Isolation of Small Interfering RNAs Using Viral Suppressors of RNA Interference Marius van den Beek, Christophe Antoniewski, and Clément Carré
14 Computational Analysis, Biochemical Purification, and Detection of tRNA-Derived Small RNA Fragments.
Simon P. Keam, Andrew Sobala, David T. Humphreys, Catherine M. Suter, and Gyorgy Hutvagner
15 Differential DNA Methylation Patterns in Endo-siRNAs Mediated Silencing of LINE-1 Retrotransposons.
Long Chen, Jane E. Dahlstrom, and Danny Rangasamy
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