Animal Cell Biotechnology 4th Edition by Ralf Pörtner ISBN 978-1071601938 1071601938

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Product details: 

ISBN 10:  1071601938

ISBN 13: 978-1071601938

Author: Ralf Pörtner

Animal Cell Biotechnology: Methods and Protocols, Fourth Edition constitutes a comprehensive manual of state-of-the-art techniques for setting up mammalian cell lines and media for development of biopharmaceuticals, and optimizing critical parameters for cell culture considering the whole cascade from the lab to the final production. Special emphasis was put on model-assisted concepts. Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in cell line and media development, optimizing process techniques and process strategies, use of model-assisted tools for process design and optimization, and in analysis. Topics include cell line and media development, techniques for process development, model-based techniques for process development, process analysis, and downstream techniques. The volume is divided into five parts that reflect the processes required for different stages of production. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Animal Cell Biotechnology: Methods and Protocols, Fourth Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.

Table of contents: 

1. Cell Line and Media Development
   Front Matter
   Near-Physiological Cell Cycle Synchronization with Countercurrent Centrifugal Elutriation
   Johannes Möller, Katrin Korte, Ralf Pörtner, An-Ping Zeng, Uwe Jandt
   Turning Cells into Reagents: The Preparation of Assay Ready Cells for Routine Use in Bioassays
   Oliver Wehmeier, Alexander Loa
   Screening of Media Supplements for High-Performance Perfusion Cultures by Design of Experiment
   Patrick Mayrhofer, Renate Kunert
2. Techniques for Process Development
   Front Matter
   ambr® 15 Microbioreactors for CHO Cell Cultivation
   Steve R. C. Warr
   Using a Parallel Micro-Cultivation System (Micro-Matrix) as a Process Development Tool for Cell Culture Applications
   Vincent Wiegmann, Cristina Bernal Martinez, Frank Baganz
   HEK293 Cell-Based Bioprocess Development at Bench Scale by Means of Online Monitoring in Shake Flasks (RAMOS and SFR)
   Tibor Anderlei, Michael V. Keebler, Jordi Joan Cairó, Martí Lecina
   Orbitally Shaken Single-Use Bioreactor for Animal Cell Cultivation: Fed-Batch and Perfusion Mode
   Tim Bürgin, Juliana Coronel, Gerrit Hagens, Michael V. Keebler, Yvonne Genzel, Udo Reichl et al.
   Development of Mammalian Cell Perfusion Cultures at Lab Scale: From Orbitally Shaken Tubes to Benchtop Bioreactors
   Moritz Wolf, Massimo Morbidelli
   Perfusion Control for High Cell Density Cultivation and Viral Vaccine Production
   Alexander Nikolay, Thomas Bissinger, Gwendal Gränicher, Yixiao Wu, Yvonne Genzel, Udo Reichl
   How to Produce mAbs in a Cube-Shaped Stirred Single-Use Bioreactor at 200 L Scale
   Cedric Schirmer, Jan Müller, Nina Steffen, Sören Werner, Regine Eibl, Dieter Eibl
3. Model-Based Techniques for Process Development
   Front Matter
   Generic Workflow for the Setup of Mechanistic Process Models
   Sven Daume, Sandro Kofler, Julian Kager, Paul Kroll, Christoph Herwig
   Estimation of Process Model Parameters
   Sahar Deppe, Björn Frahm, Volker C. Hass, Tanja Hernández Rodríguez, Kim B. Kuchemüller, Johannes Möller et al.
   Efficient Optimization of Process Strategies with Model-Assisted Design of Experiments
   Kim B. Kuchemüller, Ralf Pörtner, Johannes Möller
   Design, Optimization, and Adaptive Control of Cell Culture Seed Trains
   Tanja Hernández Rodríguez, Björn Frahm
4. Process Analysis
   Front Matter
   High-Throughput Quantification and Glycosylation Analysis of Antibodies Using Bead-Based Assays

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