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Angiogenesis Protocols Second Edition by Cliff Murray, Stewart Martin ISBN 9781597452410 1597452416

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Angiogenesis Protocols Second Edition Zubair Ahmed Bsc Digital Instant Download

Author(s): Zubair Ahmed BSC, PHD, Roy Bicknell BSC, PHD (auth.), Cliff Murray, Stewart Martin (eds.)
ISBN(s): 9781597452410, 1597452416
Edition: 2
File Details: PDF, 6.40 MB
Year: 2009
Language: english
SKU: EB-1297706 Category: Tags: , , , , ,

Angiogenesis Protocols Second Edition by Cliff Murray, Stewart Martin- Ebook PDF Instant Download/Delivery: 9781597452410, 1597452416
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Product details: 

ISBN 10:  1597452416

ISBN 13: 9781597452410

Author:  Cliff Murray, Stewart Martin 

As experimentation and clinical trials with first generation anti-angiogenic agents have yielded results and our understanding of the biology and physiology of blood and lymphatic vessels has increased, a new angiogenesis volume swiftly became a necessity. Angiogenesis Protocols, Second Edition remains true to its original vision of providing a single source for angiogenesis researchers, irrespective of levels of resource and expertise, by collecting a range of methods for cell isolation and assessing angiogenesis in vivo or in vitro. This information, however, is expanded to include chapters on circulating endothelial progenitor cells, angiogenic signalling pathways, imaging of angiogenesis, and measurement of tissue blood flow. Written in the Methods in Molecular Biology™ series format, the chapters provide step-by-step laboratory protocols, lists of necessary materials and reagents, and a Notes section, which details tips on troubleshooting and avoiding known pitfalls.

Extensive and cutting-edge, Angiogenesis Protocols, Second Edition is not only a practical handbook for key techniques, but also an informative and enjoyable read for all those interested, no matter how directly, in angiogenesis.

Table of contents: 

Angiogenic Signalling Pathways

  • Angiogenic Signalling Pathways — Zubair Ahmed, Roy Bicknell

  • Angiogenic Signalling Pathways — Natalie Charnley, Stephanie Donaldson, Pat Price

Microscopic Assessment

  • Front Matter

  • Assessing Tumor Angiogenesis in Histological Samples — Stephen B Fox

  • Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis — Steven Clasper, David G. Jackson

Cell Isolation Techniques

  • Front Matter

  • Vascular Endothelial Cells from Human Micro- and Macro-vessels: Isolation, Characterisation and Culture — Peter W Hewett

  • Lymphatic Endothelial Cells: Establishment of Primaries and Characterization of Established Lines — Riccardo E Nisato, Michael S Pepper

  • Isolation, Culture and Characterisation of Vascular Smooth Muscle Cells — Adrian T. Churchman, Richard C.M. Siow

  • Circulating Endothelial Progenitor Cells (CEPC) — Michael C. Schmid, Judith A. Varner

In vitro Techniques

  • Front Matter

  • In vitro Assays for Endothelial Cell Functions Related to Angiogenesis: Proliferation, Motility, Tubular Differentiation and Proteolysis — Suzanne A Eccles, William Court, Lisa Patterson, Sharon Sanderson

  • Tube Formation: an In Vitro Matrigel Angiogenesis Assay — M Lourdes Ponce

  • Three-dimensional In vitro Angiogenesis in the Rat Aortic Ring Model — David C West, Mike F Burbridge

  • Static and Dynamic Assays of Cell Adhesion Relevant to the Vasculature — Lynn M. Butler, Helen M. McGettrick, Gerard B. Nash

  • Genetic Manipulation of Corneal Endothelial Cells: Transfection and Viral Transduction — Eckart Bertelmann

  • Creation of Human Skin Equivalents for the In vitro Study of Angiogenesis in Wound Healing — Ira M. Herman, Alice Leung

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Tags: Cliff Murray, Stewart Martin, Angiogenesis Protocols