Germline Stem Cells 3rd Edition by Michael Buszczak ISBN 978-1071632611 1071632612

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Product details: 

ISBN 10:    1071632612

ISBN 13: 978-1071632611

Author: Michael Buszczak

This third edition volume expands on the previous editions with new discussion on the latest techniques and model systems used to study germline stem cells. The chapters in this book cover topics such as analysis of the C. elegans germline stem cell pool; live imaging of the Drosophila testis stem cell niche; standardization of single-cell RNA-sequencing analysis workflow to study Drosophila ovary; 3D analysis of the intact mouse ovary; and derivation and primordial germ cell induction of intermediate pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Germline Stem Cells: Methods and Protocols, Third Edition is a valuable resource for all researchers in the germ stem cell community interested in learning more about this developing field.

Table of contents: 

1 Analysis of the C. elegans Germline Stem Cell Pool.

Sarah L. Crittenden, Hannah S. Seidel, and Judith Kimble

2 Analysis of C. elegans Germline Small RNA Pathways.

Mathias S. Renaud, Uri Seroussi, and Julie M. Claycomb

3 Lineage Tracing and Single-Cell RNA-seq in C. elegans to Analyze Transgenerational Epigenetic Phenotypes Inherited from Germ Cells.

Juan D. Rodriguez and David J. Katz

4 Methods to Analyze Nutritional and Inter-Organ Control of Drosophila Ovarian Germline Stem Cells.

Chad Simmons, Tancia W. Bradshaw, and Alissa R. Armstrong

5 Targeting Endogenous Loci That Function in Drosophila Germline Stem Cells

Marianne Mercer, Varsha Bhargava, Courtney D. Goldstein, and Michael Buszczak

6 Live Imaging of the Drosophila Testis Stem Cell Niche

Leah J. Greenspan and Erika L. Matunis

7 Enrichment of Undifferentiated Germline and Somatic Cells from Drosophila Testes.

Sharif M. Ridwan, Matthew Antel, and Mayu Inaba

8 Spermatogonial Dedifferentiation into Germline Stem Cells in Drosophila Testes

Salvador C. Herrera and Erika A. Bach

9 Standardization of Single-Cell RNA-Sequencing Analysis Workflow to Study Drosophila Ovary

Deeptiman Chatterjee and Wu-Min Deng

10 Identification of Germline Stem Cells in Zebrafish

Bruce W. Draper

11 Characterization of the Postnatal Naked Mole-Rat Ovary: From Primordial Germ Cells to Meiotic Prophase I Oocytes…

Miguel Angel Brieño-Enríquez

12 A Roadmap for Three-Dimensional Analysis of the Intact Mouse Ovary.

Bikem Soygur, Mariko H. Foecke, Eliza A. Gaylord, Adam Fries, Jing Li, Ripla Arora, and Diana J. Laird

13 Isolation and Culture Techniques for Fetal Mouse Germ Cells

Guillaume Burnet, Josephine Bowles, and Cassy M. Spiller

14 Rattus norvegicus Spermatogenesis Colony-Forming Assays

Karen M. Chapman, Ashutosh Pudasaini, Morgan N. Vanderbeck, and F. Kent Hamra

15 Maintenance of Human Primordial Germ Cell-Like Cells in a Long-Term Culture System

Joanna J. Gell and Toshi Shioda

16 Derivation and Primordial Germ Cell Induction of Intermediate Pluripotent Stem Cells.

Leqian Yu, Emily Ballard, Charles A. Finzon Artisans, and Jun Wu

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Tags: Michael Buszczak, Germline Stem Cells